Ren Quan, Zhao Ya-Pu, Yue J C, Cui Y B
State Key Laboratory of Nonlinear Mechanics (LNM), Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, China.
Biomed Microdevices. 2006 Sep;8(3):201-8. doi: 10.1007/s10544-006-8173-9.
In this paper, construction of hybrid device by integrating nanowires with F(1)-ATPase motors is described. The nickel nanowires and multi-segment nanowires, including gold and nickel, were fabricated by electrochemical deposition in nanoporous templates. The nickel nanowires functionalized by biotinylated peptide can be assembled directly onto F(1)-ATPase motors to act as the propellers. If the multi-component nanowires, including gold and nickel, were selectively functionalized by the thiol group modified ssDNA and the synthetic peptide, respectively, the biotinylated F(1)-ATPase motors can be attached to the biotinylated peptide on nickel segment of the nanowires. Then, the multi-component nanowires can also be used as the propellers, and one may observe the rotations of the multi-component nanowires driven by F(1)-ATPase motors. Therefore, introduction of multiple segments along the length of a nanowire can lead to a variety of multiple chemical functionalities, which can be selectively bound to cells and special biomolecules. This method provides an insight for the construction of other hybrid devices with its controlling arrangement of different biomolecule on designed nanometer scale structures.
本文描述了通过将纳米线与F(1)-ATP酶马达整合来构建混合装置的过程。镍纳米线以及包括金和镍的多段纳米线是通过在纳米多孔模板中进行电化学沉积制备的。用生物素化肽功能化的镍纳米线可以直接组装到F(1)-ATP酶马达上作为推进器。如果包括金和镍的多组分纳米线分别通过硫醇基团修饰的单链DNA和合成肽进行选择性功能化，生物素化的F(1)-ATP酶马达就可以附着到纳米线镍段上的生物素化肽上。然后，多组分纳米线也可以用作推进器，人们可以观察到由F(1)-ATP酶马达驱动的多组分纳米线的旋转。因此，沿着纳米线长度引入多个片段可以产生多种化学功能，这些功能可以选择性地与细胞和特殊生物分子结合。该方法为在设计的纳米尺度结构上通过控制不同生物分子的排列来构建其他混合装置提供了思路。
