Zhang Jie, Shu Yao-Gen
Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, China.
Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing, 100190, China.
Genes Dis. 2016 Nov 25;4(1):37-40. doi: 10.1016/j.gendis.2016.11.002. eCollection 2017 Mar.
FF-ATPase is an active rotary motor, and generates three-ATP for each rotation. At saturated substrate concentration, the motor can achieve about 10 r.p.m, which means one motor can generate about 10 ATP molecules during 30 min. Here, we constituted a novel nanodevice with a molecular rotary motor and a "battery", FF-ATPase and chromatophore, and presented a novel method of sandwich type rotary biosensor based on subunit with one target-to-one motor, in which one target corresponds 10 ATP molecules as detection signals during 30 min. The target such as NT-proBNP detection demonstrated that this novel nanodevice has potential to be developed into an ultrasensitive biosensor to detect low expressed targets.
FF-ATPase是一种活性旋转马达，每旋转一圈产生三个ATP。在底物浓度饱和时，该马达转速可达约10转/分钟，这意味着一个马达在30分钟内可产生约10个ATP分子。在此，我们构建了一种由分子旋转马达和“电池”（FF-ATPase和色素细胞）组成的新型纳米装置，并提出了一种基于亚基的夹心式旋转生物传感器新方法，其中一个靶标对应一个马达，在30分钟内一个靶标对应10个ATP分子作为检测信号。对NT-proBNP等靶标的检测表明，这种新型纳米装置有潜力被开发成一种超灵敏生物传感器，用于检测低表达靶标。
