Shiomi Naofumi, Yamaguchi Yutaka, Nakai Hiroaki, Fujita Tomoko, Katsuda Tomohisa, Katoh Shigeo
Department of Biosphere Sciences, Kobe College, 4-1 Okadayama, Nishinomiya, Japan.
J Biosci Bioeng. 2006 Sep;102(3):206-9. doi: 10.1263/jbb.102.206.
The rates of degradation of cyanuric acid, a key intermediate in a metabolic pathway of s-triazine herbicides, were measured for Pseudomonas sp. NRRL B-12227. The rate of degradation was affected by the rate of cyanuric acid transport through cell membranes and the activity of cyanuric acid amidohydrolase inside the cells. At low concentrations of cyanuric acid, the acclimation of cells to cyanuric acid and/or added nutrients effectively enhanced the degradation rate. The strain was also applied to bioremediation using a Bioremediation with Self-Immobilization System (BSIS), in which Pseudomonas sp. NRRL B-12227 cells were co-immobilized with Bacillus subtilis, the latter of which secretes a viscous polymer, in a shallow layer of soil packed in a column. More than 70% of the Pseudomonas sp. NRRL B-12227 cells were co-immobilized with the B. subtilis in a 7.5 cm layer of the packed soil by self-aggregation. More than 60% of the 1 mM cyanuric acid supplied to the packed soil was degraded in this layer during a 72 h period.
测定了假单胞菌属NRRL B - 12227对氰尿酸（均三嗪类除草剂代谢途径中的关键中间体）的降解速率。降解速率受氰尿酸通过细胞膜的转运速率以及细胞内氰尿酸酰胺水解酶活性的影响。在低浓度氰尿酸条件下，细胞对氰尿酸和/或添加营养物的适应有效地提高了降解速率。该菌株还被应用于使用自固定化生物修复系统（BSIS）的生物修复，在该系统中，假单胞菌属NRRL B - 12227细胞与枯草芽孢杆菌共固定化，后者分泌一种粘性聚合物，固定在填充于柱中的浅层土壤中。超过70%的假单胞菌属NRRL B - 12227细胞通过自聚集与枯草芽孢杆菌在7.5厘米厚的填充土壤层中共固定化。在72小时内，供应给填充土壤的1 mM氰尿酸中有超过60%在该层中被降解。
