Wang B X, Chen X G, Zhang W
Department of Pharmacology, Academy of Traditional Chinese Medicine and Materia Medica of Jilin Province, Changchun.
Yao Xue Xue Bao. 1990;25(5):321-5.
The polyamines of pilose antler (PASPA) consist of putrescine (PU, 70.9%), spermidine (SPD, 26.3%) and spermine (SP, 2.8%). The incorporations of [3H] leucine into protein and [3H] uridine into RNA in mouse liver tissue were increased when PASPA was given orally to mice at the dose of 30 mg/kg for 4 successive days. The incorporations of [3H] leucine into liver protein and [3H] uridine into the cytosolic and nuclear RNA were also increased by treatment with PU (21 mg/kg). In addition, the RNA polymerase activity in the solubilized liver nuclear fraction of PU (21 mg/kg)-treated mice was increased. SPD only promoted the synthesis of protein in mouse liver tissue at the dose of 8 mg/kg. However, SP showed no effect on the synthesis of protein and RNA polymerase activity under the used dose (1 mg/kg). The results suggest that PASPA is the main active substance responsible for the promotion of the synthesis of protein and RNA in mouse liver.
鹿茸多胺（PASPA）由腐胺（PU，70.9%）、亚精胺（SPD，26.3%）和精胺（SP，2.8%）组成。当以30mg/kg的剂量连续4天给小鼠口服PASPA时，小鼠肝组织中[3H]亮氨酸掺入蛋白质和[3H]尿苷掺入RNA的量增加。用PU（21mg/kg）处理也可增加肝蛋白质中[3H]亮氨酸的掺入以及胞质和核RNA中[3H]尿苷的掺入。此外，用PU（21mg/kg）处理的小鼠肝细胞核溶解部分的RNA聚合酶活性增加。SPD仅在剂量为8mg/kg时促进小鼠肝组织中的蛋白质合成。然而，在所使用的剂量（1mg/kg）下，SP对蛋白质合成和RNA聚合酶活性没有影响。结果表明，PASPA是促进小鼠肝中蛋白质和RNA合成的主要活性物质。
