Wang B X, Chen X G, Xu H B, Zhang W, Zhang J
Department of Pharmacology, Academy of Traditional Chinese Medicine, Changchun.
Yao Xue Xue Bao. 1990;25(9):652-7.
The incorporations of [3H] leucine into protein and [3H] uridine into RNA in mouse liver were increased when PASPA was given to mice at a dose of 30 mg/kg for 4 successive days. The RNA polymerase activity, especially the RNA polymerase II activity in the solubilized liver nuclear fraction of PASPA-treated mice was also increased. In vitro experiment demonstrated that PASPA increased the RNA polymerase activity significantly in mouse liver nuclei at a concentration of 1 microgram/ml. These results suggest that the enhancement of RNA polymerase activities, particularly RNA polymerase II activity, induced by PASPA treatment is responsible for the increase in synthesis of protein and RNA in mouse liver tissue.
当以30毫克/千克的剂量连续4天给小鼠注射PASPA时，小鼠肝脏中[3H]亮氨酸掺入蛋白质和[3H]尿苷掺入RNA的量增加。PASPA处理小鼠的可溶性肝核部分中的RNA聚合酶活性，尤其是RNA聚合酶II活性也增加。体外实验表明，PASPA在浓度为1微克/毫升时可显著增加小鼠肝细胞核中的RNA聚合酶活性。这些结果表明，PASPA处理诱导的RNA聚合酶活性增强，特别是RNA聚合酶II活性增强，是小鼠肝脏组织中蛋白质和RNA合成增加的原因。
