利用生化和中子活化技术对牛肾中锌结合蛋白和镉结合蛋白的研究。

Studies on zinc- and cadmium-bound proteins in bovine kidneys by biochemical and neutron activation techniques.

作者信息

Jayawickreme C K, Chatt A

机构信息

Department of Chemistry, Dalhousie University, Halifax, NS, Canada.

出版信息

Biol Trace Elem Res. 1990 Jul-Dec;26-27:503-12. doi: 10.1007/BF02992706.

DOI:10.1007/BF02992706

PMID:1704756

Abstract

Bovine kidneys were found to contain about 78 ppm Zn and 0.78 ppm Cd. Approximately 45% of Zn and 60% of Cd were present in the cytosol fraction. More than 95% of these two metals were bound to macromolecules. Both Zn- and Cd-protein complexes were observed to be stable between pH 7 and 10.5. Separation and characterization of these proteins were carried out using several chromatographic and electrophoretic techniques in conjunction with instrumental neutron activation analysis (INAA). The results showed the presence of at least four Zn-binding proteins with mol wt greater than 300,000, 260,000, 89,000, and 27,000 and at least three Cd-binding proteins of mol wt greater than 300,000, 32,000, and 13,000.

摘要

发现牛肾含有约78 ppm的锌和0.78 ppm的镉。约45%的锌和60%的镉存在于胞质溶胶部分。这两种金属超过95%与大分子结合。观察到锌和镉的蛋白质复合物在pH 7至10.5之间是稳定的。使用几种色谱和电泳技术结合仪器中子活化分析（INAA）对这些蛋白质进行分离和表征。结果表明存在至少四种分子量大于300,000、260,000、89,000和27,000的锌结合蛋白，以及至少三种分子量大于300,000、32,000和13,000的镉结合蛋白。