Sato M, Nagai Y
Arch Environ Contam Toxicol. 1989 Jul-Aug;18(4):587-93. doi: 10.1007/BF01055026.
The effects of zinc (Zn) deficiency and repeated exposure to cadmium (Cd) on the accumulation and distribution of metallothionein (MT), Cd and Zn in the liver and kidney were studied. Male Sprague-Dawley rats were fed either a Zn-deficient (1 ppm) or a Zn-adequate (40 ppm) diet during the experiment, and the rats were injected subcutaneously with a cadmium chloride solution (1.0 mg Cd/kg of body weight, 5 days a week) for 4 weeks. Cadmium, Zn, and Cd-induced MT concentrations in the liver and kidney were lower in the Zn-deficient rats (-Zn + Cd) than in the Zn-adequate rats (+Zn + Cd), while the content of Cd bound to high molecular weight proteins (HMWP) was greater in the Zn-deficient rats (-Zn + Cd). The Zn bound to Cd-induced MT was reduced to 30% in the liver and to 60% in the kidney of the Zn-deficient rats (-Zn + Cd) as compared with that of the Zn-adequate rats (+Zn + Cd). In the kidney of Zn-deficient rats, exposure to Cd caused a decrease in essential Zn associated with HMWP as compared with that of Zn-adequate rats (+Zn + Cd). Thus, Zn-deficiency affected the distribution of Cd in tissues, MT and HMWP and accelerated substantially Cd-induced Zn-deficiency in the kidney.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了锌（Zn）缺乏和反复接触镉（Cd）对肝脏和肾脏中金属硫蛋白（MT）、镉和锌的积累与分布的影响。实验期间，将雄性斯普拉格-道利大鼠分为两组，分别喂食锌缺乏（1 ppm）或锌充足（40 ppm）的饮食，并且每周5天皮下注射氯化镉溶液（1.0 mg Cd/ kg体重），持续4周。锌缺乏的大鼠（-Zn + Cd）肝脏和肾脏中镉、锌以及镉诱导的MT浓度低于锌充足的大鼠（+Zn + Cd），而锌缺乏的大鼠（-Zn + Cd）中与高分子量蛋白质（HMWP）结合的镉含量更高。与锌充足的大鼠（+Zn + Cd）相比，锌缺乏的大鼠（-Zn + Cd）肝脏中与镉诱导的MT结合的锌减少至30%，肾脏中减少至60%。在锌缺乏大鼠的肾脏中，与锌充足的大鼠（+Zn + Cd）相比，接触镉导致与HMWP相关的必需锌减少。因此，锌缺乏影响了镉在组织、MT和HMWP中的分布，并显著加速了镉诱导的肾脏锌缺乏。（摘要截短至250字）
