Brandão Aline Dias, Viccini Lyderson Facio, Salimena Fátima Regina Gonçalves, Vanzela André Luiz Laforga, Recco-Pimentel Shirlei Maria
Departamento de Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13084-971, Campinas, SP, Brazil.
J Plant Res. 2007 Mar;120(2):317-21. doi: 10.1007/s10265-006-0041-4. Epub 2006 Oct 18.
The aim of this work was to determine the cytogenetic characteristics of Brazilian Lippia alba (Mill) N. E. Brown and Lantana camara Plum. that could be useful for future characterization of these genera. Our analyses revealed that Li. alba has 2n=30 chromosomes consisting of ten metacentric and five submetacentric pairs, while La. camara has 44 metacentric chromosomes. The large blocks of heterochromatin seen in both species suggest an apomorphic condition. Six 45S rDNA sites were detected in both species by fluorescence in situ hybridization (FISH). Two and four 5S rDNA sites were observed in Li. alba and La. camara, respectively. Meiotic analysis revealed a normal chromosomal behaviour. The number of chromosomes and the presence of 45S rDNA and 5S rDNA sites do not exclude a possible polyploid origin. The cytogenetic differences between La. camara and Li. alba may be useful markers for differentiating these species.
这项工作的目的是确定巴西的白香蜂草（唇形科）和马缨丹的细胞遗传学特征，这可能有助于将来对这些属进行特征描述。我们的分析表明，白香蜂草有2n = 30条染色体，由十对中着丝粒染色体和五对亚中着丝粒染色体组成，而马缨丹有44条中着丝粒染色体。在这两个物种中都观察到的大片异染色质表明其为一种衍征状态。通过荧光原位杂交（FISH）在这两个物种中均检测到六个45S rDNA位点。在白香蜂草和马缨丹中分别观察到两个和四个5S rDNA位点。减数分裂分析显示染色体行为正常。染色体数目以及45S rDNA和5S rDNA位点的存在并不排除可能的多倍体起源。马缨丹和白香蜂草之间的细胞遗传学差异可能是区分这些物种的有用标记。
