Bellon-Fontaine M N, Mozes N, van der Mei H C, Sjollema J, Cerf O, Rouxhet P G, Busscher H J
Laboratoire de génie de l'hygiène et des procédés alimentaires, Institute National de la Recherche Agronomique, Massy, France.
Cell Biophys. 1990 Aug;17(1):93-106. doi: 10.1007/BF02989805.
Four different thermodynamic approaches were compared on their usefulness to predict correctly the adhesion of two fouling microogranisms from dairy processing to various solid substrata. The surface free energies of the interacting surfaces were derived from measured contact angles according to: 1. The equation of state; 2. The geometric-mean equation using dispersion and polar components neglecting spreading pressures; 3. The geometric-mean equation using dispersion and polar components while accounting for spreading pressures; and 4. The Lifshitz-van der Waals/Acid-Base approach. All approaches yielded similar surface free energies for the low energy surfaces. Application of approach 1 with different liquids did not give consistent values for the high surface free energy substrata. The dispersion or Lifshiftz-van der Waals components were nearly equal for approaches 2, 3, and 4; however, the polar or acid-base components differed greatly according to the approach followed. Approaches 1 and 2 correctly predicted that adhesion should occur, although the trend with respect to the various solid substrata was opposite the one experimentally observed, as was also the trend predicted by approach 4. Only approach 3 correctly predicted the observed bacterial adhesion with respect to the various solid substrata. In approach 3 and 4, adhesion was frequently found, despite a positive free energy of adhesion. This was attributed to either possible local attractive electrostatic interactions, inadequate weighing of surface free energy components in the calculation of free energies of adhesion, or to additional forces arising from structured interfacial water.
比较了四种不同的热力学方法，以评估它们正确预测两种来自乳制品加工过程中的污垢微生物在各种固体基质上的粘附情况的有效性。相互作用表面的表面自由能根据以下方法从测量的接触角推导得出：1. 状态方程；2. 使用色散和极性成分且忽略铺展压力的几何平均方程；3. 使用色散和极性成分并考虑铺展压力的几何平均方程；4. Lifshitz-范德华力/酸碱方法。对于低能表面，所有方法得出的表面自由能相似。对于高表面自由能基质，使用方法1并结合不同液体时，得到的值并不一致。方法2、3和4的色散或Lifshiftz-范德华力成分几乎相等；然而，极性或酸碱成分根据所采用的方法有很大差异。方法1和2正确预测了应该会发生粘附，尽管相对于各种固体基质的趋势与实验观察到的相反，方法4预测的趋势也是如此。只有方法3正确预测了在各种固体基质上观察到的细菌粘附情况。在方法3和4中，尽管粘附自由能为正，但仍经常发现有粘附现象。这归因于可能存在的局部吸引静电相互作用、在计算粘附自由能时对表面自由能成分的权衡不足，或者是由结构化界面水产生的额外力。
