Schulze Katja, Koch Annette, Petri-Fink Alke, Steitz Benedikt, Kamau Sarah, Hottiger Michael, Hilbe Monika, Vaughan Lloyd, Hofmann Margarethe, Hofmann Heinrich, von Rechenberg Brigitte
Musculoskeletal Research Unit, Equine Hospital, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
J Nanosci Nanotechnol. 2006 Sep-Oct;6(9-10):2829-40. doi: 10.1166/jnn.2006.484.
Superparamagnetic iron oxide nanoparticles (SPION) were coated with either Polyvinyl alcohol (PVA) or Vinyl alcohol/vinyl amine copolymer and further functionalized with the fluorochromes Cy3.5 or Texas Red. A colloidally stable suspension of nanoparticles was incubated on sheep synovial cells in vitro for 3, 24, 72, and 120 hours. Nanoparticle internalization into synoviocytes as well as biocompatibility was visualized using light, fluorescence and confocal microscopy and fluorochrome labeled cells were quantified by flow cytometry. Data were analyzed by ANOVA factorial tests. Amino-PVA-SPION alone was detectable in cytoplasmic endosome-like structures after 3 hours of incubation but resulted in early cell death after 24 hours. Although amino-PVA-Cy3.5-SPION and PVA-TexasRed-SPION were taken up more slowly and less intensely, both labeled more than 80% of the cells in culture, but did not significantly change cell morphology or vitality at any time of evaluation in comparison to control cells. Results indicate that functionalized amino PVA-coated SPION are biocompatible, were successfully internalized by synoviocytes and hold promise for future biomedical applications utilizing magnetic drug targeting in joint disease.
超顺磁性氧化铁纳米颗粒（SPION）用聚乙烯醇（PVA）或乙烯醇/乙烯胺共聚物进行包被，并用荧光染料Cy3.5或德克萨斯红进一步功能化。将纳米颗粒的胶体稳定悬浮液在体外与绵羊滑膜细胞孵育3、24、72和120小时。使用光学显微镜、荧光显微镜和共聚焦显微镜观察纳米颗粒内化到滑膜细胞中的情况以及生物相容性，并通过流式细胞术对荧光染料标记的细胞进行定量分析。数据通过方差分析析因检验进行分析。单独的氨基-PVA-SPION在孵育3小时后可在细胞质内体样结构中检测到，但在24小时后导致早期细胞死亡。尽管氨基-PVA-Cy3.5-SPION和PVA-德克萨斯红-SPION摄取较慢且强度较低，但两者均标记了培养物中超过80%的细胞，并且与对照细胞相比，在任何评估时间均未显著改变细胞形态或活力。结果表明，功能化的氨基PVA包被的SPION具有生物相容性，成功地被滑膜细胞内化，并有望在未来利用磁靶向药物治疗关节疾病的生物医学应用中发挥作用。
