Hellstern Daniel, Schulze Katja, Schöpf Bernhard, Petri-Fink Alke, Steitz Benedikt, Kamau Sarah, Hilbe Monika, Koch-Schneidemann Sabine, Vaughan Lloyd, Hottiger Michael, Hofmann Margarethe, Hofmann Heinrich, von Rechenberg Brigitte
Musculoskeletal Research Unit, Equine Hospital, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
J Nanosci Nanotechnol. 2006 Sep-Oct;6(9-10):3261-8. doi: 10.1166/jnn.2006.482.
PVA coated and fluorescent dye (Cy3.5) functionalized vinyl alcohol/vinyl amine copolymer coated superparamagnetic iron oxide nanoparticles (SPION) were evaluated for systemic distribution and elimination after intraarticular injection in sheep. Observation was done at 3, 24, 72, and 120 hours after injection using light microscopy, fluorescent microscopy, and confocal microscopy. No pathologic influence of SPION on the tissue harvested could be seen. A significantly increased iron content could be identified in the kidneys, lymph nodes, and spleen after injection of SPION. No particles were detected in the liver, the urinary, and the gall bladder. No positive fluorescent signal could be attributed to SPION throughout the organs. Our results indicated that the iron component of the SPION is possible to be incorporated into the physiologic iron metabolism after reabsorption in the proximal tubule system of the kidney and that concentration levels of Cy3.5 are too low to be detected throughout the body.
对聚乙烯醇（PVA）包被且用荧光染料（Cy3.5）功能化的乙烯醇/乙烯胺共聚物包被的超顺磁性氧化铁纳米颗粒（SPION），在绵羊关节内注射后进行全身分布和清除情况评估。在注射后3小时、24小时、72小时和120小时，使用光学显微镜、荧光显微镜和共聚焦显微镜进行观察。未发现SPION对所采集组织有病理影响。注射SPION后，在肾脏、淋巴结和脾脏中可检测到铁含量显著增加。在肝脏、泌尿系统和胆囊中未检测到颗粒。在所有器官中均未检测到可归因于SPION的阳性荧光信号。我们的结果表明，SPION中的铁成分在肾脏近端小管系统重吸收后有可能纳入生理铁代谢，并且Cy3.5的浓度水平过低，无法在全身检测到。
