苏郁胶囊对抑郁模型小鼠行为及海马神经元损伤的影响

[Effect of suyu capsule on behavior and injury of hippocampal neurons in depression model mice].

作者信息

Zhong Xiao-Ming, Mao Qing-Qiu, Huang Zhen, Wei Jia-Ping, Liang Ze-Hua

机构信息

Department of Pharmacy, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2006 Jul;31(14):1192-5.

PMID:17048593

Abstract

OBJECTIVE

To observe the effect of Suyu capsule on behavior, injury of hippocampal neurons and Ca2+ ion in hippocampal synaptic in the depression model mice.

METHOD

Sixty male Kunming mice were randomly divided into 5 groups, the control group, the model group and three Suyu capsule groups (the doses were 22.8, 11.4, 5.7 g x kg(-1) respectively). The model was established by separation and chronic unpredictable mild stimulation. The increased weight and crossing score, rearing score were measured by open-field and sweet water consumption of mice. Cone cell and configuration of neuron in CA1, CA3 region of hippocampus were observed by Nissl. The concentration of hippocampal synaptic Ca2+ ion was detected by fluorimetry.

RESULT

Comparing with the mice of control, the increased weight was slowered ( P < 0.01), the scores of rearing and crossing were decreased (P < 0.01), sweet water consumption were decreased too (P < 0.01), numbers of cone cell in CA3 region of hippocampus were decreased obviously (P < 0.01), and Ca2+ ion in hippocampal synaptic was increased obviously. Comparing with the mice of model, Suyu capsule (22.8 g kg(-1)) could increase the increased weight on the 14th and 21 st day obviously (P < 0.05); Suyu capsule (22.8 g x kg(-1)) could increase the scores of crossing obviously (P < 0.05), Suyu capsule (22.8, 11.4 g x kg(-1)) could increase the scores of rearing obviously (P < 0.01, P < 0.05); Suyu capsule (22.8, 11.4, 5.8 g x kg(-1)) could increase sweet water consumption obviously (P < 0.01, P < 0.05, P < 0.05; Suyu capsule (22.8, 11.4, 5.8 g x kg(-1)) could increase numbers of cone cell in CA3 region of hippocampus obviously (P < 0.01, P < 0.05, P < 0.05); Suyu capsule (22.8, 11.4, 5.8 g x kg(-1)) decreased Ca2+ ion in hippocampal synaptic with dose-effect relationship (P < 0.01, P < 0.01, P < 0.05).

CONCLUSION

Suyu capsule can improve all the symptoms of the depression model mice and protect injury of hippocampal neurons in the depression model mice. The possible mechanism of action is to restrict Ca2+ ion overfreight.

摘要

目的

观察苏郁胶囊对抑郁模型小鼠行为、海马神经元损伤及海马突触Ca2+离子的影响。

方法

60只雄性昆明小鼠随机分为5组，即对照组、模型组和3个苏郁胶囊组（剂量分别为22.8、11.4、5.7 g·kg-1）。采用隔离和慢性不可预知温和刺激法建立模型。通过旷场实验测量小鼠体重增加量、穿越得分、直立得分，以及通过糖水消耗实验测量小鼠糖水消耗量。采用尼氏染色法观察海马CA1、CA3区锥体细胞及神经元形态。采用荧光分析法检测海马突触Ca2+离子浓度。

结果

与对照组小鼠相比，模型组小鼠体重增加缓慢（P<0.01），直立得分和穿越得分降低（P<0.01），糖水消耗量减少（P<0.01），海马CA3区锥体细胞数量明显减少（P<0.01），海马突触Ca2+离子明显增加。与模型组小鼠相比，苏郁胶囊（22.8 g·kg-1）可明显增加第14天和21天的体重增加量（P<0.05）；苏郁胶囊（22.8 g·kg-1）可明显增加穿越得分（P<0.05），苏郁胶囊（22.8、11.4 g·kg-1）可明显增加直立得分（P<0.01，P<0.05）；苏郁胶囊（22.8、11.4、5.8 g·kg-1）可明显增加糖水消耗量（P<0.01，P<0.05，P<0.05）；苏郁胶囊（22.8、11.4、5.8 g·kg-1）可明显增加海马CA3区锥体细胞数量（P<0.01，P<0.05，P<0.05）；苏郁胶囊（22.8、11.4、5.8 g·kg-1）可使海马突触Ca2+离子减少，呈剂量效应关系（P<0.01，P<0.01，P<0.05）。