Du Xue-han, Jiang Li, Jiang Xiao-qing
The Clinical Medical School of Southeast University, Nanjing 210009, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(11):921-3.
To study the therapeutic mechanisms of Danshen injection in intrahepatic cholestasis of pregnancy (ICP).
The experimental serum was collected from 6 patients with ICP before therapy when in hospital. Endothelial cells were isolated from human umbilical veins chosen among the 10 normal pregnancy according to Jaffe's method. Serum of 20% concentration of ICP and different concentration of Danshen injection were cocultured with human vascular endothelial cells (HUVECs) for 24 hours. MTT method was applied to measuring the vitality of HUVECs. The expression value of vascular endothelial growth factor (VEGF) of different groups was detected by immunocytochemistry and enzyme-linked immunosorbent assay (ELISA) respectively.
Compared with ICP group, the value of MTT and VEGF in the Danshen injection therapeutic groups were increased with the increase of concentration and moreover, 8 g x L(-1) of Danshen injection was the optical concentration. By immunocytochemistry, the expression of VEGF in HUVECs in the Danshen injection therapeutic groups was also enhanced with the increase of concentration.
Danshen injection can protect HUVECs against the injury of ICP serum and promote the expression of VEGF. Danshen injection improves HUVECs by increasing the value of VEGF.
研究丹参注射液治疗妊娠肝内胆汁淤积症（ICP）的作用机制。
收集6例ICP患者住院治疗前的实验血清。按照贾菲法从10例正常妊娠者中选取人脐静脉分离内皮细胞。将20%浓度的ICP血清及不同浓度的丹参注射液与人血管内皮细胞（HUVECs）共培养24小时。采用MTT法检测HUVECs的活力。分别通过免疫细胞化学法和酶联免疫吸附测定（ELISA）法检测不同组血管内皮生长因子（VEGF）的表达值。
与ICP组相比，丹参注射液治疗组的MTT值和VEGF值随浓度增加而升高，且丹参注射液8 g·L⁻¹为最佳浓度。通过免疫细胞化学法检测，丹参注射液治疗组HUVECs中VEGF的表达也随浓度增加而增强。
丹参注射液可保护HUVECs免受ICP血清的损伤并促进VEGF的表达。丹参注射液通过增加VEGF值改善HUVECs。
