Xiao Bao-hong, Zhang Chun-ling, Wu Xiao
Department of Respiration, Qingdao Central Hospital, Qingdao 266042, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(12):1002-5.
To observe the inhibitory effects of crescent euphorbia on tumor growth, immunoregulation, the side effect on the mice blood system, cell cycle and to investigate its effect on apoptosis of tumor cells.
Crescent euphorbia was administered gastrally to C57BL/6J mouse implanted with Lewis lung cancer for 12 days. Inhibition on tumor growth, immunoregulation,the side effect on the mice blood system and cell cycle were observed and its effect on apoptosis of lung cancer cells were investigated.
(1) Inhibitory rates of crescent euphorbia at doses of 7.5, 15, 30 g x kg(-1) and 60 g x kg(-1) were 0.61%, 16.93%, 32.81% and 58.26% respectively, significant differenel with those of controls at doses of 30 g x kg(-1) and 60 g x kg(-1)(P < 0.05). (2) Although no effect on thymus index in normal mouse at all dose of crescent euphorbia was found, spleen index in tumor-bear mice was reduced significantly at dose of 60 g x kg(-1)(P < 0.05). (3) No side effect on the miceblood system was found. (4) The percentage of S phase of cell cycle was increased in the group of crescent euphorbia at dose of 30 g x kg(-1), 60 g x kg(-1) and the apoptotic rate was (16.43 +/- 18.69)% and (24.37 +/- 15.48)% by flow eytometry (P < 0.05). The apoptotic index (AI)of these two groups were (4.00 +/- 7.50)% and (5.93 +/- 5.96)% by TUNEL, however apoptosis was found in the group at doses of 60 g kg(-1) (P < 0.01).
Crescent euphorbia can inhibit the growth of Lewis lung cancer in mice, no side effection for the mice blood system and the effect might be associated with induction of apoptosis on cancer cells.
观察泽漆对肿瘤生长的抑制作用、免疫调节作用、对小鼠血液系统的副作用及细胞周期的影响，并探讨其对肿瘤细胞凋亡的作用。
对接种Lewis肺癌的C57BL/6J小鼠灌胃给予泽漆12天。观察其对肿瘤生长的抑制作用、免疫调节作用、对小鼠血液系统的副作用及细胞周期的影响，并探讨其对肺癌细胞凋亡的作用。
（1）7.5、15、30 g·kg⁻¹和60 g·kg⁻¹剂量的泽漆抑瘤率分别为0.61%、16.93%、32.81%和58.26%，30 g·kg⁻¹和60 g·kg⁻¹剂量组与对照组相比差异有统计学意义（P < 0.05）。（2）各剂量泽漆对正常小鼠胸腺指数均无影响，但60 g·kg⁻¹剂量可使荷瘤小鼠脾脏指数显著降低（P < 0.05）。（3）对小鼠血液系统无副作用。（4）30 g·kg⁻¹和60 g·kg⁻¹剂量泽漆组细胞周期S期百分比升高，流式细胞术检测凋亡率分别为（16.43±18.69）%和（24.37±15.48）%（P < 0.05）；TUNEL法检测这两组凋亡指数（AI）分别为（4.00±7.50）%和（5.93±5.96）%，60 g·kg⁻¹剂量组可见凋亡（P < 0.01）。
泽漆可抑制小鼠Lewis肺癌生长，对小鼠血液系统无副作用，其作用可能与诱导癌细胞凋亡有关。
