Huang Sheng-Teng, Yang Rong-Chi, Lee Pei-Nir, Yang Su-Hui, Liao Shuen-Kuei, Chen Tzu-Ya, Pang Jong-Hwei S
Graduate Institute of Clinical Medical Sciences, Chang Gung University, Tao-Yuan, Taiwan, ROC.
Int Immunopharmacol. 2006 Jun;6(6):870-9. doi: 10.1016/j.intimp.2005.12.010. Epub 2006 Jan 30.
Phyllanthus urinaria, a widely used herb medicine in Asia, was tested for its anti-tumor effect in vivo for the first time. The anti-tumor activity in P. urinaria extract was evaluated by its effect on tumor developed in C57BL/6J mice with implantation of Lewis lung carcinoma cells. The oral administration of P. urinaria to mice caused significant inhibition of tumor development with lower occurrence rate and markedly reduced tumor size. Neither the total body weight of mouse nor the weights of organs including heart, lung, liver, spleen and kidney revealed any difference between two groups, suggesting limited in vivo cytotoxic effect of P. urinaria in mice. TUNEL assay demonstrated the increase of apoptosis in tumor sections prepared from P. urinaria-treated mice compared with control mice. It is worth of note that the neovascularization in tumor was inhibited in P. urinaria-treated mice, which implicated the potential anti-angiogenic effect of P. urinaria. Further study using an in vitro matrix-induced tube formation of HUVECs again confirmed the anti-angiogenic action of P. urinaria. P. urinaria exerted no inhibitory effect on the growth of HUVECs, however, the migration of HUVECs as analyzed using transwell assay was suppressed markedly by P. urinaria in a dose-dependent manner. All together, the present study indicated that P. urinaria extract is an anti-tumor and anti-angiogenic agent, which can be used safely in animals.
叶下珠是一种在亚洲广泛使用的草药，首次在体内测试其抗肿瘤作用。通过其对植入Lewis肺癌细胞的C57BL/6J小鼠体内肿瘤的影响来评估叶下珠提取物的抗肿瘤活性。给小鼠口服叶下珠可显著抑制肿瘤发展，发生率降低，肿瘤大小明显减小。两组小鼠的总体重以及包括心脏、肺、肝脏、脾脏和肾脏在内的器官重量均未显示出任何差异，这表明叶下珠在小鼠体内的细胞毒性作用有限。TUNEL检测表明，与对照小鼠相比，用叶下珠处理的小鼠制备的肿瘤切片中凋亡增加。值得注意的是，用叶下珠处理的小鼠肿瘤中的新血管形成受到抑制，这暗示了叶下珠潜在的抗血管生成作用。使用体外基质诱导人脐静脉内皮细胞（HUVECs）形成管状物的进一步研究再次证实了叶下珠的抗血管生成作用。叶下珠对HUVECs的生长没有抑制作用，然而，用transwell检测分析发现，叶下珠以剂量依赖性方式显著抑制HUVECs的迁移。总之，本研究表明叶下珠提取物是一种抗肿瘤和抗血管生成剂，可在动物中安全使用。
