Shen Yun-hui, Chen Chang-xun, Xu Zhen-ye
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine.
Zhongguo Zhong Yao Za Zhi. 2006 May;31(9):754-9.
To investigate the leukogenic function of Shuanghuang Shengbai (SHSB) granule and the related mechanisms.
Mouse leukopenic models were induced by radiation. Mice were divided into normal control group, model control group, positive control group-Li kejun tablet group and three different dose (high, middle, low-dose) groups of SHSB granule. The peripheral hemogram, thymus index (TI), spleen index (SI), bone marrow nucleated cell (BMNC) and colony forming unit-spleen (CFU-S) were evaluated. The proliferation of bone marrow cells was determined. The in vitro cultured colony forming unit granulocyte macrophage (CFU-GM) was estimated. The index of CD34+ cell in BMNC were determined by flow cytometry. The ultra-micro structure of bone marrow were observed by electromicroscope.
(1)SHSB rranule could increase the WBC of model mice; (2)SHSB granule could increase BMNC and promote the proliferation of bone marrow cell; (3)SHSB granule could increase CFU-S, CFU-GM and CD34+ cell index in BMNC of model mice significantly; (4)SHSB Granule could also protect the bone marrow hemotopoietic microenvironment from the harm of radiation; (5)SHSB granule could increase the SI of model mice, indicating the enhancement of immunological function.
SHSB granule has apparent leukogenic function. The mechanism may be related to enhancing the proliferation of hematopoietic cells and protecting the bone marrow hemotopoietic microenvironment.
探讨双黄升白（SHSB）颗粒的升白作用及其相关机制。
采用辐射诱导小鼠白细胞减少模型。将小鼠分为正常对照组、模型对照组、阳性对照组-利可君片组以及SHSB颗粒高、中、低三个不同剂量组。检测外周血象、胸腺指数（TI）、脾脏指数（SI）、骨髓有核细胞（BMNC）及脾集落形成单位（CFU-S）。测定骨髓细胞增殖情况。评估体外培养的粒-巨噬细胞集落形成单位（CFU-GM）。采用流式细胞术检测BMNC中CD34+细胞指数。通过电镜观察骨髓超微结构。
（1）SHSB颗粒可提高模型小鼠白细胞计数；（2）SHSB颗粒可增加BMNC数量并促进骨髓细胞增殖；（3）SHSB颗粒可显著提高模型小鼠BMNC中的CFU-S、CFU-GM及CD34+细胞指数；（4）SHSB颗粒还可保护骨髓造血微环境免受辐射损伤；（5）SHSB颗粒可提高模型小鼠的SI，提示免疫功能增强。
SHSB颗粒具有明显的升白作用。其机制可能与增强造血细胞增殖及保护骨髓造血微环境有关。
