Liu Wei, Zhang Zhao-qin, Zhao Xiao-min, Gao Yun-sheng
The 88th Hospital of PLA, Tai'an, China.
Zhongguo Zhong Yao Za Zhi. 2006 May;31(9):763-5.
To investigate the effect of Uncaria rhynchophylla total alkaloids (RTA) pretreatment on the voltage-gated sodium currents of the rat hippocampal neurons after acute hypoxia.
Primary cultured hippocampal neurons were divided into RTA pre-treated and non-pretreated groups. Patch clamp whole-cell recording was used to compare the voltage-gated sodium current amplitude and threshold with those before hypoxia.
After acute hypoxia, sodium current amplitude was significantly decreased and its threshold was upside. RTA pretreatment could inhibit the reduction of sodium current amplitude.
RTA pretreatment alleviates the acute hypoxia-induced change of sodium currents, which may be one of the mechanisms for protective effect of RTA on cells.
探讨钩藤总碱（RTA）预处理对大鼠海马神经元急性缺氧后电压门控钠电流的影响。
原代培养的海马神经元分为RTA预处理组和未预处理组。采用膜片钳全细胞记录法比较缺氧前后电压门控钠电流的幅度和阈值。
急性缺氧后，钠电流幅度显著降低，阈值上移。RTA预处理可抑制钠电流幅度的降低。
RTA预处理可减轻急性缺氧诱导的钠电流变化，这可能是RTA对细胞产生保护作用的机制之一。
