使用D标记的N-乙基马来酰亚胺和13C标记的碘代乙酰苯胺通过基质辅助激光解吸/电离飞行时间质谱进行定量蛋白质组分析。
Quantitative proteome analysis using D-labeled N-ethylmaleimide and 13C-labeled iodoacetanilide by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
作者信息
Kurono Sadamu, Kurono Tamie, Komori Naoka, Niwayama Satomi, Matsumoto Hiroyuki
机构信息
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA.
出版信息
Bioorg Med Chem. 2006 Dec 15;14(24):8197-209. doi: 10.1016/j.bmc.2006.09.023. Epub 2006 Oct 16.
Abstract
A new methodology for quantitative analysis of proteins is described, applying stable-isotope labeling by small organic molecules combined with one- or two-dimensional electrophoresis and MALDI-TOF-MS, also allowing concurrent protein identification by peptide mass fingerprinting. Our method eliminates fundamental problems in other existing isotope-tagging methods requiring liquid chromatography and MS/MS, such as isotope effects, fragmentation, and solubility. It is also anticipated to be more practical and accessible than those LC-dependent methods.
摘要
本文描述了一种蛋白质定量分析的新方法，该方法将小分子稳定同位素标记与一维或二维电泳及基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）相结合，还可通过肽质量指纹图谱同时进行蛋白质鉴定。我们的方法消除了其他现有需要液相色谱和串联质谱的同位素标记方法中存在的基本问题，如同位素效应、碎片化和溶解性问题。预计该方法也比那些依赖液相色谱的方法更实用、更易操作。
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