Chien Tzu-I, Lu Jin-Ying, Kao Jau-Tsuen, Lee Tai-Fen, Ho Shu-Yuan, Chang Chia-Yu, Liu Chao-Wei, Lee Chun-Hsien, Yan Shiao-Ni, Yang Jui-Yi, Lin Po-Chuang, Wang Mei-Ju
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Clin Chim Acta. 2007 Feb;377(1-2):98-102. doi: 10.1016/j.cca.2006.08.033. Epub 2006 Sep 5.
Our study is aimed to determine the performance of 3 automated urinalysis systems-Clinitek Atlas, Urisys 2400 and Aution Max.
One thousand urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing urine chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria.
The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively.
The automated urinalysis systems demonstrate acceptable correlations with each other in urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary urine culture.
我们的研究旨在确定3种自动尿液分析系统——Clinitek Atlas、Urisys 2400和Aution Max的性能。
使用这3种自动系统对1000份尿液标本进行分析。比较这3种检测方法在尿液化学检测以及评估白细胞酯酶和亚硝酸盐检测菌尿能力方面的结果。
对于pH值、血液、葡萄糖、尿胆原、酮体和比重，Atlas系统与Aution Max系统之间的相关性在1个分级差异内表现更好。对于蛋白质和亚硝酸盐，Atlas系统与Urisys 2400之间观察到更好的相关性，而对于胆红素和白细胞，Aution Max与Urisys 2400之间的相关性更好。Clinitek Atlas、Aution Max和Urisys 2400检测白细胞酯酶和亚硝酸盐筛查显著菌尿的敏感性和特异性分别为71.7%、58.9%、70.8%和99.1%、99.1%、97.2%。
自动尿液分析系统在尿液化学检测方面相互之间表现出可接受的相关性，尤其是Clinitek Atlas系统和Aution Max系统在大多数项目上。这3种仪器检测白细胞酯酶和亚硝酸盐筛查显著菌尿的特异性和阴性预测值足以避免不必要的尿液培养。
