黑色素瘤分化相关基因5（mda-5）而非视黄酸诱导基因I（RIG-I）是副粘病毒V蛋白的常见靶点。

mda-5, but not RIG-I, is a common target for paramyxovirus V proteins.

作者信息

Childs Kay, Stock Nicola, Ross Craig, Andrejeva Jelena, Hilton Louise, Skinner Michael, Randall Richard, Goodbourn Stephen

机构信息

Division of Basic Medical Sciences, St. George's, University of London, London SW17 0RE, UK.

出版信息

Virology. 2007 Mar 1;359(1):190-200. doi: 10.1016/j.virol.2006.09.023. Epub 2006 Oct 16.

DOI:10.1016/j.virol.2006.09.023

PMID:17049367

Abstract

The induction of IFN-beta by the paramyxovirus PIV5 (formerly known as SV5) is limited by the action of the viral V protein that targets the cellular RNA helicase mda-5. Here we show that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mda-5. The inhibition of IFN-beta induction is not species-restricted, being observed in a range of mammalian cells as well as in avian cells, and we show that the inhibition of mda-5 function is also not restricted to mammalian cells. In contrast, the V proteins do not bind to the related RNA helicase RIG-I and do not inhibit its activity. The relative contributions of mda-5 and RIG-I to IFN-beta induction are discussed.

摘要

副粘病毒PIV5（以前称为SV5）对IFN-β的诱导作用受到靶向细胞RNA解旋酶mda-5的病毒V蛋白的限制。我们在此表明，其他12种副粘病毒也通过其V蛋白保守的富含半胱氨酸的C末端与mda-5解旋酶结构域之间的直接相互作用来靶向mda-5。IFN-β诱导的抑制作用不受物种限制，在一系列哺乳动物细胞以及禽类细胞中均能观察到，并且我们表明mda-5功能的抑制也不限于哺乳动物细胞。相比之下，V蛋白不与相关的RNA解旋酶RIG-I结合，也不抑制其活性。文中讨论了mda-5和RIG-I对IFN-β诱导的相对贡献。