Gopalakrishnapai Jayashree, Gupta Garima, Karthikeyan T, Sinha Sharmistha, Kandiah Eaazhisai, Gemma Emiliano, Oscarson Stefan, Surolia Avadhesha
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
Biochem Biophys Res Commun. 2006 Dec 8;351(1):14-20. doi: 10.1016/j.bbrc.2006.09.164. Epub 2006 Oct 9.
Earlier we established using modeling studies the residues in calreticulin (CRT) important for sugar-binding (M. Kapoor, H. Srinivas, K. Eaazhisai, E. Gemma, L. Ellgaard, S. Oscarson, A. Helenius, A. Surolia, Interactions of substrate with calreticulin, an endoplasmic reticulum chaperone, J. Biol. Chem. 278 (8) (2003) 6194-6200). Here, we discuss the relative roles of Trp-319, Asp-317, and Asp-160 for sugar-binding by using site-directed mutagenesis and isothermal titration calorimetry (ITC). Residues corresponding to Asp-160 and Asp-317 in CNX play important role towards sugar-binding. From the present study we demonstrate that the residue Asp-160 is not involved in sugar-binding, while Asp-317 plays a crucial role. Further, it is also validated that cation-pi interactions of the sugar with Trp-319 dictate sugar-binding in CRT. This study not only defines further the binding site of CRT but also highlights its subtle differences with that of calnexin.
