Davey Mark W, Keulemans Johan, Swennen Rony
Laboratory for Fruit Breeding and Biotechnology, Division of Crop Biotechnics, Catholic University of Leuven, De Croylaan 42, Heverlee, Leuven, Belgium.
J Chromatogr A. 2006 Dec 15;1136(2):176-84. doi: 10.1016/j.chroma.2006.09.077. Epub 2006 Oct 17.
As part of a screening program to identify micronutrient-rich banana and plantain (Musa) varieties, a simple, robust, and comparatively rapid protocol for the quantification of the provitamin A carotenoids contents of fruit pulp and peel tissues by HPLC and by spectrophotometry has been developed. Major points to note include the use lyophilisation and extensive tissue disruption procedures to ensure quantitative recoveries, and the avoidance of saponification and/or concentration steps which lead to significant losses of provitamin A carotenoids. The protocol showed excellent reproducibility between replicate extractions, without the need for an internal standard. Application of the methodology demonstrated that Musa fruit pulp has a relatively simple provitamin A carotenoids content, quite different from the overlying peel, and that the proportions of alpha- and beta-carotene are characteristic for each genotype. The protocol was also used to profile the provitamin A carotenoids of several other fruits.
作为一项旨在鉴定富含微量营养素的香蕉和大蕉（芭蕉属）品种的筛选计划的一部分，已开发出一种简单、可靠且相对快速的方案，用于通过高效液相色谱法（HPLC）和分光光度法对果肉和果皮组织中的维生素A原类胡萝卜素含量进行定量。需要注意的要点包括使用冻干法和广泛的组织破碎程序以确保定量回收，以及避免导致维生素A原类胡萝卜素大量损失的皂化和/或浓缩步骤。该方案在重复提取之间显示出极好的重现性，无需内标。该方法的应用表明，芭蕉属水果的果肉中维生素A原类胡萝卜素含量相对简单，与上层果皮有很大不同，并且α-胡萝卜素和β-胡萝卜素的比例对每个基因型来说都是独特的。该方案还用于分析其他几种水果的维生素A原类胡萝卜素。
