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[使用不同型号的光电比色计测定悬浮液的分散度]

[Determination of the dispersity of suspensions using photoelectric colorimeters of different makes].

作者信息

Cherniakhovskaia I V, Tul'bovich E V, Khramina V P

出版信息

Lab Delo. 1990(11):53-4.

PMID:1704960
Abstract

Different conditions of measuring the suspension dispersity of photoelectric colorimeters of two brands,KFK-2 and FEK-56, result in distorted data: the dispersity index (DI) measured by KFK according to the method developed for FEK is inaccurate. The presence of 2 photoreceptors in the KFK apparatus, in contrast to the FEK one, results in elevated values of optic density (D) when the apparatus sensitivity is changed, starting from 590 nm wavelength (this fact is mentioned in the operation instructions) and eventually breaks the inversely proportional relationships between the D value and wavelength. DI estimation is based on the proportional relationships between wavelengths and D values obtained with a selected light filter pair. The wavelength selection mode had to be changed in the procedure of DI measurement by KFK-2 (or by KFK-MP) so that the light filter pair was placed in the same sensitivity zone; the distance between the cuvette and photo element was also changed (the cuvette with the sample was placed not in the cuvette holder but at a maximum distance from the photo element, closer to the source of light). Such modifications helped obtain compatible results of optic density measurements and DI values with photoelectric colorimeters of various brands. This is true only for suspensions rather than for transparent solutions, which fact is explained by the theory of light dispersion by suspension particles.

摘要

测量两个品牌KFK - 2和FEK - 56光电比色计悬浮液分散度的不同条件会导致数据失真:KFK按照为FEK开发的方法测量的分散度指数(DI)不准确。与FEK仪器不同,KFK仪器中有2个光感受器,当仪器灵敏度发生变化时,从波长590 nm开始,光密度(D)值会升高(这一事实在操作说明中有提及),最终打破了D值与波长之间的反比例关系。DI估计基于使用选定滤光片对获得的波长与D值之间的比例关系。在KFK - 2(或KFK - MP)测量DI的过程中,必须改变波长选择模式,以便滤光片对处于相同的灵敏度区域;比色皿与光电元件之间的距离也发生了变化(装有样品的比色皿不是放在比色皿架中,而是放在离光电元件最远的位置,更靠近光源)。这些修改有助于使用不同品牌的光电比色计获得兼容的光密度测量结果和DI值。这仅适用于悬浮液,而不适用于透明溶液,这一事实由悬浮颗粒的光散射理论解释。

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