[血清中唾液酸的测定]
[Determination of sialic acids in blood serum].
作者信息
Skorniakov V I, Saiapin A V, Kozhemiakin L A
出版信息
Lab Delo. 1989(2):32-4.
The blood serum sialic acid concentrations are recommended to be measured according to G. Sydow (1985) with the estimation of the results from the mean molar (E) and molar (E) extinction coefficients without using N-acetylneuraminic acid as the reference value. The E value for devices emitting polychromatic light (FEK-56M, KFK, KFK-2, SPECOL 211) is 132 sm-1.M-1, that for devices emitting monochromatic light (SF-4A), E = 138 sm-1.M-1. The results obtained by this method correlate with those of Hess' method in conditional units. The method is simple, no acetic acid is necessary for it, the stained complex formed is stable.
建议根据G. Sydow（1985年）的方法测量血清唾液酸浓度，通过平均摩尔（E）和摩尔（E）消光系数估算结果，且不使用N-乙酰神经氨酸作为参考值。发射多色光的设备（FEK-56M、KFK、KFK-2、SPECOL 211）的E值为132 sm⁻¹·M⁻¹，发射单色光的设备（SF-4A）的E值为138 sm⁻¹·M⁻¹。用该方法得到的结果与Hess方法在条件单位下的结果相关。该方法简单，无需乙酸，形成的染色复合物稳定。
Zotero 插件