Katori Hideaki, Nozawa Akinori, Tsukuda Mamoru
Department of Otolaryngology, Yokohama City Minato Red Cross Hospital, 3-12-1 Shin-yamashita, Naka-ku, Yokohama 231-8682, Japan.
Auris Nasus Larynx. 2007 Mar;34(1):79-84. doi: 10.1016/j.anl.2006.09.006. Epub 2006 Oct 17.
In the present study, we attempted to identify cyclooxygenase-2 (COX-2) expression and Ki-67 index in carcinoma ex-pleomorphic adenoma (Ca ex-PA) using quantitative immunohistochemical analysis and to compare the benign component of the neoplasia. We also aimed to relate the overexpression of COX-2 with the pathways of malignant transformation of Ca ex-PA as evidenced by distinct morphological features.
Forty Ca ex-PA from patients treated at Department of Otolaryngology, Yokohama City University Medical Center, Yokohama, Japan, from 1999 to 2005, were selected. All Ca ex-PA showed only one malignant histological component: adenocarcinoma (23 cases), adenoid-cystic carcinoma (10), epithelial-myoepithelial carcinoma (7). The tissues were stained with monoclonal antibodies to COX-2 and Ki-67. The results were analyzed using quantitative immunohistochemical analysis. We also analyzed the association of the histological classification of the carcinomatous component.
In the immunohistochemical analysis of COX-2 and Ki-67 index, significant increase was observed in Ca ex-PA, especially with adenocarcinoma, compared to pleomorphic adenoma and sialadenitis. Quantitative assessment is more sensitive as a measure of cellular protein content as compared to standard optical density measurement.
The data support the hypothesis that increased COX-2 expression is associated with early events in malignant transformation of pleomorphic adenoma.
在本研究中，我们试图通过定量免疫组织化学分析确定癌在多形性腺瘤（癌在多形性腺瘤，Ca ex-PA）中的环氧合酶-2（COX-2）表达和Ki-67指数，并比较肿瘤的良性成分。我们还旨在将COX-2的过表达与Ca ex-PA恶性转化途径相关联，这一点可通过不同的形态学特征得到证实。
选取1999年至2005年在日本横滨市立大学医学中心耳鼻喉科接受治疗的患者的40例Ca ex-PA。所有Ca ex-PA仅显示一种恶性组织学成分：腺癌（23例）、腺样囊性癌（10例）、上皮-肌上皮癌（7例）。组织用抗COX-2和Ki-67单克隆抗体染色。结果采用定量免疫组织化学分析进行分析。我们还分析了癌性成分的组织学分类之间的关联。
在COX-2和Ki-67指数的免疫组织化学分析中，与多形性腺瘤和涎腺炎相比，Ca ex-PA中观察到显著增加，尤其是腺癌。与标准光密度测量相比，定量评估作为细胞蛋白含量的一种测量方法更敏感。
数据支持这样的假设，即COX-2表达增加与多形性腺瘤恶性转化的早期事件相关。
