Börmer O P, Thrane-Steen K
Central Laboratory, Norwegian Radium Hospital, Oslo.
Tumour Biol. 1991;12(1):9-15. doi: 10.1159/000217682.
We have mapped the epitope group specificity of the monoclonal antibodies employed in six commercial sandwich immunometric assays for carcinoembryonic antigen (CEA), using cross-inhibition experiments with monoclonal antibodies previously classified in an International Workshop on the Epitope Reactivity of Monoclonal Antibodies against CEA. In this workshop it was shown that most monoclonal antibodies could be classified into one of five epitope groups, designated Gold groups 1-5. We found that of the six assay kits tested, five used solid-phase antibody of Gold group 4. Three of these (CIS ELSA 2-CEA, Hybritech Tandem-R CEA, Roche CEA EIA Duomab 60) used labeled antibody of Gold group 1, the remaining two (Abbott CEA-RIA Monoclonal, Wallac/LKB DELFIA CEA) used antibodies of Gold group 5. The sixth assay kit (Behringwerke Enzygnost CEA) used a solid-phase antibody of Gold group 1, and a polyclonal labeled antibody.
我们利用与先前在癌胚抗原（CEA）单克隆抗体表位反应性国际研讨会上分类的单克隆抗体进行交叉抑制实验，绘制了用于六种商业化癌胚抗原夹心免疫分析检测的单克隆抗体的表位组特异性图谱。在该研讨会上表明，大多数单克隆抗体可分为五个表位组之一，即戈尔德组1-5。我们发现，在所测试的六种检测试剂盒中，五种使用戈尔德组4的固相抗体。其中三种（CIS ELSA 2-CEA、Hybritech Tandem-R CEA、罗氏CEA EIA Duomab 60）使用戈尔德组1的标记抗体，其余两种（雅培CEA-RIA单克隆、Wallac/LKB DELFIA CEA）使用戈尔德组5的抗体。第六种检测试剂盒（贝林werke Enzygnost CEA）使用戈尔德组1的固相抗体和多克隆标记抗体。
