Medeiros L J, Herrington R D, Gonzalez C L, Jaffe E S, Cossman J
Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Am J Clin Pathol. 1991 Mar;95(3):363-8. doi: 10.1093/ajcp/95.3.363.
The My4 antibody, one of a number of monoclonal antibodies that react with the CD14 antigen, was originally reported to weakly stain monocytes, macrophages, and granulocytes. However, recent studies have shown that the My4 antibody also stains normal peripheral blood B lymphocytes and some subtypes of B-cell non-Hodgkin's lymphoma. Thus, the authors have studied a large series of non-Hodgkin's lymphomas stained with the My4 antibody. In frozen sections of reactive lymph node biopsy specimens, the My4 antibody strongly stained mantle zone B lymphocytes and weakly reacted with dendritic reticulum cells and histiocytes. In a series of 245 non-Hodgkin's lymphomas, the My4 antibody stained 111 (45%) cases: 108 of 189 (57%) B-cell lymphomas, 3 of 50 (6%) T-cell lymphomas, and 0 of 6 null cell lymphomas. My4-positive B-cell lymphomas occurred in all histologic subtypes with the exception of small noncleaved cell lymphomas. Follicular lymphomas were most often My4 positive (82%). My4 antibody staining showed no correlation with Working Formulation grade. All three My4-positive T-cell lymphomas had a mature T-cell phenotype. Seventy-six of the 111 (68%) My4-positive lymphomas were also analyzed with at least one other anti-CD14 antibody, either Mo2 and/or Leu-M3. In all cases the antigens that react with Mo2 and Leu-M3 were not expressed. Thus, the staining of reactive and neoplastic B cells by My4 appears to be unique to this antibody and is not a feature of all anti-CD14 antibodies.
My4抗体是与CD14抗原发生反应的多种单克隆抗体之一，最初报道其对单核细胞、巨噬细胞和粒细胞的染色较弱。然而，最近的研究表明，My4抗体也可对正常外周血B淋巴细胞和某些B细胞非霍奇金淋巴瘤亚型进行染色。因此，作者对大量用My4抗体染色的非霍奇金淋巴瘤进行了研究。在反应性淋巴结活检标本的冰冻切片中，My4抗体强烈染色套区B淋巴细胞，与树突状网状细胞和组织细胞反应较弱。在一系列245例非霍奇金淋巴瘤中，My4抗体染色111例（45%）：189例B细胞淋巴瘤中的108例（57%）、50例T细胞淋巴瘤中的3例（6%）、6例无细胞淋巴瘤中的0例。除小无裂细胞淋巴瘤外，所有组织学亚型中均出现My4阳性B细胞淋巴瘤。滤泡性淋巴瘤最常呈My4阳性（82%）。My4抗体染色与工作分类分级无关。所有3例My4阳性T细胞淋巴瘤均具有成熟T细胞表型。111例My4阳性淋巴瘤中的76例（68%）也用至少一种其他抗CD14抗体（Mo2和/或Leu-M3）进行了分析。在所有病例中，与Mo2和Leu-M3反应的抗原均未表达。因此，My4对反应性和肿瘤性B细胞的染色似乎是该抗体所特有的，并非所有抗CD14抗体的特征。
