Dorfman D M, Shahsafaei A
Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.
Mod Pathol. 1996 Oct;9(10):959-65.
Specimens of lymphoid and splenic tissue with reactive changes or involvement by non-Hodgkin's lymphomas or Hodgkin's disease were examined for low-affinity nerve growth factor receptor (NGFR) immunoreactivity of dendritic reticulum cells (DRCs) in frozen tissue and paraffin sections. The DRCs in 13 of 13 specimens with reactive follicular hyperplasia were uniformly immunoreactive with the NGFR-specific antibody NGFR5 and with the DRC-specific antibody DRC-1. We also found that the DRCs associated with 30 of 30 cases of follicular non-Hodgkin's lymphoma were immunoreactive with NGFR5 in a pattern similar to that seen with DRC-1, in contrast to a previous study. Our results were similar in frozen tissue and microwave-treated paraffin sections. Four of four cases of diffuse large cell non-Hodgkin's lymphoma, six of six cases of immunoblastic large cell non-Hodgkin's lymphoma and three of three cases of small noncleaved cell non-Hodgkin's lymphoma did not exhibit significant tumor-associated NGFR5-positive DRCs. Similarly, no NGFR5 staining was observed in eight of eight cases of small lymphocytic lymphoma/chronic lymphocytic leukemia or in six of six cases of marginal zone lymphoma, except in residual germinal centers. However, in 10 of 11 cases of mantle cell lymphoma, the DRCs present in a loose tumor-associated meshwork were reactive with NGFR5; this finding is of potential utility in the differential diagnosis of low-grade lymphoproliferative disorders. In four of four cases of nodular lymphocyte predominance Hodgkin's disease, tumor nodule-associated DRCs were immunoreactive for NGFR in a pattern similar to that seen with DRC-1, but NGFR-positive DRCs were not observed in association with other types of Hodgkin's disease. These results indicate that NGFR expression by DRCs is not lost in follicular non-Hodgkin's lymphomas and mantle cell lymphomas. Nerve growth factor and NGFR may have a role in the function of DRCs and the formation of the DRC matrix in normal and neoplastic lymphoid follicles and in other DRC-associated neoplasms.
对具有反应性改变或伴有非霍奇金淋巴瘤或霍奇金病累及的淋巴组织和脾组织标本，在冰冻组织和石蜡切片中检测树突状网状细胞（DRC）的低亲和力神经生长因子受体（NGFR）免疫反应性。13例反应性滤泡增生标本中的DRC均与NGFR特异性抗体NGFR5和DRC特异性抗体DRC-1呈免疫反应。与之前的一项研究相反，我们还发现30例滤泡性非霍奇金淋巴瘤病例中的DRC与NGFR5呈免疫反应，其模式与DRC-1相似。我们在冰冻组织和微波处理的石蜡切片中的结果相似。4例弥漫性大细胞非霍奇金淋巴瘤、6例免疫母细胞性大细胞非霍奇金淋巴瘤和3例小无裂细胞非霍奇金淋巴瘤均未显示出明显的肿瘤相关NGFR5阳性DRC。同样，在8例小淋巴细胞淋巴瘤/慢性淋巴细胞白血病或6例边缘区淋巴瘤中，除残留生发中心外，其余均未观察到NGFR5染色。然而，在11例套细胞淋巴瘤中的10例中，存在于疏松肿瘤相关网络中的DRC与NGFR5呈反应性；这一发现对低级别淋巴增殖性疾病的鉴别诊断具有潜在的实用价值。在4例结节性淋巴细胞为主型霍奇金病中，肿瘤结节相关的DRC与NGFR呈免疫反应，其模式与DRC-1相似，但在其他类型的霍奇金病中未观察到NGFR阳性DRC。这些结果表明，在滤泡性非霍奇金淋巴瘤和套细胞淋巴瘤中，DRC的NGFR表达并未丧失。神经生长因子和NGFR可能在正常和肿瘤性淋巴滤泡以及其他与DRC相关的肿瘤中DRC的功能和DRC基质的形成中发挥作用。
