Sargent N S, Habib F K
University Department of Surgery (WGH), Western General Hospital, Edinburgh, Scotland.
J Steroid Biochem Mol Biol. 1991 Jan;38(1):73-7. doi: 10.1016/0960-0760(91)90403-r.
Human hyperplastic prostate tissue was homogenised in high ionic strength buffer and the post nuclear homogenate was incubated with 0.8% octyl glucoside and bovine brain lipids. Dialysis of the resulting liposome suspension yielded a preparation in which 5 alpha-reductase was active and stable for at least three weeks and showed an increase in specific activity (Vmax +/- SD = 48.9 +/- 7.4 pmol DHT/mg protein/ml) over that of the starting homogenate (Vmax +/- SD = 5.6 +/- 1.5 pmol DHT/mg protein/min) of 8.7 times.
将人增生性前列腺组织在高离子强度缓冲液中匀浆，然后将核后匀浆与0.8%辛基葡糖苷和牛脑脂质一起孵育。对所得脂质体悬浮液进行透析，得到一种制剂，其中5α-还原酶具有活性且至少三周内稳定，并且比起始匀浆（Vmax±SD = 5.6±1.5 pmol DHT/mg蛋白质/分钟）的比活性（Vmax±SD = 48.9±7.4 pmol DHT/mg蛋白质/毫升）提高了8.7倍。
