Palin M F, Faguy M, LeHoux J G, Pelletier G
Agriculture and Agri-Food Canada, Dairy and Swine Research and Development Centre, Lennoxville, Quebec.
Endocrine. 1998 Aug;9(1):65-9. doi: 10.1385/ENDO:9:1:65.
The pathogenesis of benign prostatic hyperplasia is linked to the accumulation of dihydrotestosterone (DHT), the active form of testosterone (T), in prostatic tissue. We have defined characteristics of 5alpha-reductase enzyme which catalyzes the conversion of T into DHT in prostatic microsomes of growing pigs. Peaks for the 5alpha-reductase activity were found at pH 5.5 and 8.0, which indicates the presence of both type 1 and type 2 isozymes. Kinetic parameters of porcine 5alpha-reductase in the presence of Serenoa repens extracts revealed uncompetitive, noncompetitive, and mixed types of inhibitions. Our results show the inhibitory action of S. repens on prostate porcine microsomal 5alpha-reductase activity.
良性前列腺增生的发病机制与双氢睾酮（DHT，睾酮（T）的活性形式）在前列腺组织中的蓄积有关。我们已明确了在生长猪前列腺微粒体中催化T转化为DHT的5α-还原酶的特性。在pH 5.5和8.0时发现了5α-还原酶活性的峰值，这表明同时存在1型和2型同工酶。在锯叶棕提取物存在的情况下，猪5α-还原酶的动力学参数显示出非竞争性、非竞争性和混合型抑制作用。我们的结果表明锯叶棕对猪前列腺微粒体5α-还原酶活性具有抑制作用。
