[支气管哮喘患儿TH细胞的极化与免疫调节]

[Polarization and immune regulation of TH cells in children with bronchial asthma].

作者信息

Jiang Yu-Hong, Wang Ya-Qiu, Sun Kuan-Zhou

机构信息

Women and Children's Medical Center of Qingdao, Qingdao, Shandong 266011, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2006 Oct;8(5):379-81.

PMID:17052395

Abstract

OBJECTIVE

To study the polarization and immune regulation of TH cells in patients with bronchial asthma.

METHODS

Thirty-eight hospitalized children with bronchial asthma (ranging from 4-12 years old) and 29 age-matched healthy children (Control group) were enrolled in this study. Serum IL-2 and IFN-gamma levels were detected using ELISA. The percentage of TH1 and TH2 cells was detected by intracellular staining.

RESULTS

The serum levels of IL-2 (15.94 +/- 3.07 microg/L) and IFN-gamma (487.2 +/- 43.6 pg/mL ) in asthmatic patients were significantly lower than those in the Control group (24.73 +/- 4.37 microg/L and 654.07 +/- 14.64 pg/mL respectively; P < 0.01). The percentage of TH1 in asthmatic patients decreased significantly compared with that in the Control group [(11.24 +/- 2.43)% vs (16.67 +/- 2.73)%; P < 0.01]; in contrast, the percentage of TH2 increased compared with that in the Control group [(19.85 +/- 4.46)% vs (16.08 +/- 6.17)%; P < 0.05].

CONCLUSIONS

The serum levels of IL-2 and IFN-gamma, and the number of TH1 cells decreased in asthmatic patients. The decreased number of TH1 cells and the ratio of TH1/TH2 suggest an abnormal polarization of TH1 and TH2 cells. The changes may be associated with the inhibition of cellular immune function in asthmatic patients.

摘要

目的

研究支气管哮喘患者TH细胞的极化和免疫调节。

方法

本研究纳入了38例住院的支气管哮喘儿童（年龄4 - 12岁）和29例年龄匹配的健康儿童（对照组）。采用ELISA法检测血清白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）水平。通过细胞内染色检测TH1和TH2细胞的百分比。

结果

哮喘患者血清IL-2水平（15.94±3.07μg/L）和IFN-γ水平（487.2±43.6pg/mL）显著低于对照组（分别为24.73±4.37μg/L和654.07±14.64pg/mL；P < 0.01）。与对照组相比，哮喘患者TH1细胞百分比显著降低[（11.24±2.43）%对（16.67±2.73）%；P < 0.01]；相反，TH2细胞百分比与对照组相比升高[（19.85±4.46）%对（16.08±6.17）%；P < 0.05]。