[过敏性紫癜患儿树突状细胞产生白细胞介素-12的水平及TH1/TH2平衡的变化]

[Levels of IL-12 produced by dendritic cells and changes of TH1/TH2 balance in children with Henoch-Schonlein purpura].

作者信息

Sun Da-Qing, Zhang Qiu-Ye, Dong Zeng-Yi, Bai Feng

机构信息

Department of Pediatrics, Affiliated Hospital, Qingdao University Medical College, Qingdao 266003, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2006 Aug;8(4):307-10.

PMID:16923364

Abstract

OBJECTIVE

To study the changes of IL-12 produced by dendritic cells in peripheral blood in children with Henoch-Schonlein purpura (HSP), and to explore its influence on TH1/TH2 balance in order to elucidate its significance in the pathogenesis of HSP.

METHODS

The levels of interferon-gamma (IFN-gamma), interleukin-4 (IL-4) and interleukin-12 (IL-12) in plasma were determined by ELISA in 60 HSP children (HSP group) and 21 healthy children (Control group). Peripheral blood mononuclear cells (PBMC) of 22 HSP patients and 21 healthy children were cultured in vitro and then were transformed into dendritic cells. The levels of IL-12 in the supernatant were detected by ELISA and the positive expression rate of CD1a(+) was detected by indirect immunofluorescence procedure.

RESULTS

The levels of IFN-gamma and the ratio of IFN-gamma/IL-4 in plasma of the HSP group were lower than those of the Control group (IFN-gamma 30.59 +/- 11.27 pg/mL vs 43.38 +/- 19.19 pg/mL; IFN-gamma/IL-4 ratio 0.70 +/- 0.28 vs 1.33 +/- 0.57) (P < 0.01). The levels of IL-12 in the HSP group were also lower than those of the Control group (153.95 +/- 91.88 pg/mL vs 323.06 +/- 162.34 pg/mL; P < 0.01). In contrast, the levels of IL-4 were higher than those of the Control group (45.08 +/- 9.19 pg/mL vs 32.95 +/- 7.10 pg/mL; P < 0.01). The plasma levels of IL-12 positively correlated with the IFN-gamma levels (r=0.52, P < 0.01) and the ratio of IFN-gamma/IL-4 (r=0.43, P < 0.01) in the HSP group. 2) The IL-12 levels in the supernatant of the HSP group were lower than those of the Control group (357.06 +/- 153.56 pg/mL vs 489.80 +/- 213.45 pg/mL; P < 0.05), and had a positive correlation with the plasma IL-12 levels (r=0.74, P < 0.01). 3) The positive expression rate of CD1a(+) of the HSP group was lower than that of the Control group [(27.42 +/- 10.75)% vs (35.68 +/- 12.18)%; P < 0.05], and positively correlated with the IL-12 levels in the supernatants (r=0.57, P < 0.01) and in plasma (r=0.68, P < 0.01).

CONCLUSIONS

There was an imbalance of TH1/TH2 in HSP children. The decrease of TH1 function had a positive correlation with the low levels of IL-12 in plasma, while the latter correlated closely with decreased number and / or function of dendritic cells, suggesting that the decreased number and / or function of dendritic cells in peripheral blood resulted in the imbalance of TH1/TH2 indirectly.

摘要

目的

研究过敏性紫癜（HSP）患儿外周血中树突状细胞产生的白细胞介素-12（IL-12）的变化，探讨其对TH1/TH2平衡的影响，以阐明其在HSP发病机制中的意义。

方法

采用酶联免疫吸附测定法（ELISA）检测60例HSP患儿（HSP组）和21例健康儿童（对照组）血浆中γ-干扰素（IFN-γ）、白细胞介素-4（IL-4）和白细胞介素-12（IL-12）的水平。将22例HSP患者和21例健康儿童的外周血单个核细胞（PBMC）进行体外培养，然后诱导其分化为树突状细胞。采用ELISA法检测培养上清液中IL-12的水平，间接免疫荧光法检测CD1a(+)的阳性表达率。

结果

1）HSP组血浆中IFN-γ水平及IFN-γ/IL-4比值低于对照组（IFN-γ：30.59±11.27 pg/mL对43.38±19.19 pg/mL；IFN-γ/IL-4比值：0.70±0.28对1.33±0.57）（P<0.01）。HSP组IL-12水平也低于对照组（153.95±91.88 pg/mL对323.06±162.34 pg/mL；P<0.01）。相反，HSP组IL-4水平高于对照组（45.08±9.19 pg/mL对32.95±7.10 pg/mL；P<0.01）。HSP组血浆IL-12水平与IFN-γ水平（r=0.52，P<0.01）及IFN-γ/IL-4比值（r=0.43，P<0.01）呈正相关。2）HSP组培养上清液中IL-12水平低于对照组（357.06±153.56 pg/mL对489.80±213.45 pg/mL；P<0.05），且与血浆IL-12水平呈正相关（r=0.74，P<0.01）。3）HSP组CD1a(+)阳性表达率低于对照组[（27.42±10.75）%对（35.68±12.18）%；P<0.05]，且与培养上清液中IL-12水平（r=0.57，P<0.01）及血浆IL-12水平（r=0.68，P<0.01）呈正相关。