调节性 T 细胞和 Th1/Th2 在外周血中的变化及其在小儿哮喘中的作用

Regulatory T cells and Th1/Th2 in peripheral blood and their roles in asthmatic children.

机构信息

Clinical Laboratory, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210009, China.

出版信息

Transl Pediatr. 2013 Jan;2(1):27-33. doi: 10.3978/j.issn.2224-4336.2012.04.03.

DOI:10.3978/j.issn.2224-4336.2012.04.03

PMID:26835281

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4728940/

Abstract

OBJECTIVE

To determine the changes in CD4(+)CD25(+)Foxp3(+) regulatory T cells (Treg) and the Th1/Th2 ratio in peripheral blood of children with asthma, in order to investigate its association with asthma.

METHODS

A total of 150 children with asthma were allocated into the acute phase group (n=94) and remission phase group (n=56) based on clinical manifestations. The acute phase group was subdivided into the mild group (n=54) and severe group (n=40). Flow cytometry was applied to determine CD4(+)CD25(+)Foxp3(+) Treg, CD4(+)IFN-γ(+) Th1 and CD4(+)IL-4(+) Th2 levels in peripheral blood of different groups, and the results were compared with normal children (control group, n=50).

RESULTS

The Treg level was significantly lower in the asthma group than the control group (P<0.01): the Treg level was significantly lower in the acute phase group than the remission phase group and control group (P<0.01) and also significantly lower in the severe group than the mild group (P<0.01). The Th1/Th2 ratio was significantly lower in the asthma group than the control group (P<0.01) and also significantly lower in the acute phase group than the remission phase group and control group (P<0.01). The Treg level in peripheral blood of asthmatic children was negatively correlated with the severity of asthma (r=-0.737, P<0.01) and the Th1/Th2 ratio was also negatively correlated with the severity of asthma (r=-0.615, P<0.01), but the Treg level was positively correlated with the Th1/Th2 ratio (r=0.856, P<0.01).

CONCLUSIONS

The significantly decreased level of Treg in peripheral blood and Th subset imbalance in asthmatic children suggest the important roles of Treg and Th immunity in pathogenesis of asthma. The Treg level and Th1/Th2 ratio in peripheral blood can be used to evaluate the severity asthma.

摘要

目的

观察哮喘患儿外周血 CD4+CD25+Foxp3+调节性 T 细胞（Treg）及 Th1/Th2 比值的变化，探讨其与哮喘发病的关系。

方法

根据临床表现将 150 例哮喘患儿分为急性期组（94 例）和缓解期组（56 例），其中急性期组又分为轻症组（54 例）和重症组（40 例）。采用流式细胞术检测各组患儿外周血 CD4+CD25+Foxp3+Treg、CD4+IFN-γ+Th1 和 CD4+IL-4+Th2 水平，并与正常儿童（对照组，50 例）比较。

结果

哮喘组患儿 Treg 水平显著低于对照组（P<0.01），急性期组患儿 Treg 水平显著低于缓解期组和对照组（P<0.01），重症组患儿 Treg 水平显著低于轻症组（P<0.01）。哮喘组患儿 Th1/Th2 比值显著低于对照组（P<0.01），急性期组患儿 Th1/Th2 比值显著低于缓解期组和对照组（P<0.01）。哮喘患儿外周血 Treg 水平与哮喘严重程度呈负相关（r=-0.737，P<0.01），Th1/Th2 比值与哮喘严重程度亦呈负相关（r=-0.615，P<0.01），而 Treg 水平与 Th1/Th2 比值呈正相关（r=0.856，P<0.01）。