Wu G, Xi X, Li P, Chu X, Ruan C
Jiangsu Institute of Hematology, Suzhou Medical College, People's Republic of China.
Nouv Rev Fr Hematol (1978). 1990;32(4):231-5.
To identify the changes on the platelet plasma membrane after platelet activation, we prepared monoclonal antibodies specific for activated platelets. Hybridoma cell line SZ-51 was screened for antibodies which bound to thrombin activated platelets but not to resting platelets. 125I-SZ-51, and IgG1, bound 780 molecules/platelet on resting platelets. However, it bound 11,000 molecules/platelet on thrombin activated platelets with high affinity (4.2 nmol/L). The isolated protein using affinity chromatography showed a single band in both periodic acid-Schiff and Coomassie blue staining. Immunoblot analysis revealed that SZ-51 reacted with a 140,000 molecular weight protein which was identified to be alpha-granule membrane protein (GMP-140) in radioimmunoassay. These results demonstrate that the antigen recognized by SZ-51 is GMP-140, which is expressed on the plasma membrane of activated platelets.
为了确定血小板激活后其质膜上的变化，我们制备了针对活化血小板的单克隆抗体。对杂交瘤细胞系SZ - 51进行筛选，寻找能与凝血酶激活的血小板结合但不与静息血小板结合的抗体。125I - SZ - 51和IgG1在静息血小板上每血小板结合780个分子。然而，它在凝血酶激活的血小板上以高亲和力（4.2 nmol/L）每血小板结合11,000个分子。使用亲和层析分离的蛋白质在过碘酸希夫染色和考马斯亮蓝染色中均显示为单一条带。免疫印迹分析显示，SZ - 51与一种分子量为140,000的蛋白质反应，该蛋白质在放射免疫测定中被鉴定为α - 颗粒膜蛋白（GMP - 140）。这些结果表明，SZ - 51识别的抗原是GMP - 140，其在活化血小板的质膜上表达。
