Tanoue K, Akamatsu N, Suzuki H, Katagiri Y, Yamazaki H, Fujimoto T, Fukuda S, Onozawa Y
Dept. of Cardiovascular Res., Tokyo Metropolitan Inst. of Medical Science.
Nihon Rinsho. 1992 Feb;50(2):330-5.
The clinical significance and detection methods for circulating activated platelets are reviewed. It has been recently demonstrated that three kinds of platelet granules, dense granule, alpha-granule and lysosome, have specific membrane proteins; granulophysin, GMP-140 (PADGEM) and CD63 antigen, respectively, and that these specific granule-membrane proteins become exposed on the surface of the activated platelets. It is believed that detection of circulating activated platelets, using monoclonal antibodies specific to these granule-membrane, may be reliable and diagnostic value in thrombotic and prethrombotic diseases. Preliminary clinical studies using 2T60, a newly developed anti-GMP-140 monoclonal antibody, was presented.
本文综述了循环活化血小板的临床意义及检测方法。最近的研究表明，三种血小板颗粒，即致密颗粒、α颗粒和溶酶体，分别具有特异性膜蛋白；颗粒膜蛋白、GMP-140（血小板活化依赖颗粒外膜蛋白）和CD63抗原，并且这些特异性颗粒膜蛋白会暴露在活化血小板表面。人们认为，使用针对这些颗粒膜的单克隆抗体检测循环活化血小板，在血栓形成和血栓前疾病中可能具有可靠的诊断价值。文中介绍了使用新开发的抗GMP-140单克隆抗体2T60进行的初步临床研究。
