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[链球菌的血清学筛查。两种玻片凝集法的比较,一种仅用链球菌溶血素,另一种用几种酶(链激酶)]

[Serological screening of streptococci. Comparison of 2 methods of slide agglutination, one with streptolysin alone, the other with several enzymes (Streptozymes)].

出版信息

Pathol Biol (Paris). 1975 Sep;23(7):575-83.

PMID:170573
Abstract

Using 100 sera randomly selected, the clinical characteristis of which were known, we compared two methods of rapid detection of streptococcal infections by slide agglutination. One was the classical latex test for detection of anti-streptolysin O, the other was the streptozyme test. The latter reagent detects the presence of 5 streptococcal antibodies, anti-streptolysin O, anti-streptokinase, anti-streptohyaluronidase, anti-desoxyribonuclease and anti-nicotinamide-adenine-dinucleotidase. It was found to be more sensitive than the latex test, for antistreptolysin O. Estimations of A.S.K. and A.S.H. confirmed also the greater specificity of the streptozyme test and showed that, thanks to the presence of supplementary enzymes, it may be positive earlier than the A.S.O. latex test. Finally, used as a semi-quantitative method, streptozyme is useful to follow up approximately in the laboratory, the course of streptococcal infections.

摘要

我们使用100份临床特征已知的随机选取的血清,比较了两种通过玻片凝集法快速检测链球菌感染的方法。一种是检测抗链球菌溶血素O的经典乳胶试验,另一种是链激酶酶试验。后一种试剂可检测5种链球菌抗体的存在,即抗链球菌溶血素O、抗链激酶、抗链球菌透明质酸酶、抗脱氧核糖核酸酶和抗烟酰胺腺嘌呤二核苷酸酶。结果发现,对于抗链球菌溶血素O,它比乳胶试验更敏感。对A.S.K.和A.S.H.的评估也证实了链激酶酶试验具有更高的特异性,并且表明,由于存在补充酶,它可能比A.S.O.乳胶试验更早呈阳性。最后,作为一种半定量方法,链激酶酶试验在实验室中对于大致跟踪链球菌感染的病程很有用。

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