Alouf J E, de Saint Martin J, Eyquem A, Geoffroy C, Jacquemot C, Duphot M
Ann Microbiol (Paris). 1978 May-Jun;129(4):447-72.
A microhaemagglutination test in disposable U plates has been devised for rapid, quantitative evaluation in antistreptococcal antibodies in human sera. Fresh or freeze-dried glutaraldehyde-treated sheep erythrocytes sensitized with over fifteen extracellular proteins released by group A Streptococcus pyogenes including streptolysin O, deoxyribonucleases, hyaluronatelyase, streptokinase and nicotinamide dinucleotide glycohydrolase were used. Haemagglutination and anti-streptolysin O (ASLO) titers were determined in parallel on 434 serum specimens from 123 healthy subjects ("controls") and 311 patients with a history of supposed or evident streptococcal infection. The titration of the four above-mentioned anti-enzyme antibodies has also been made on about 100 sera from both groups. Haemagglutination titre (HT) was less than 800 in control sera. By contrast it was greater than 800 up to 12 800 in patients specimens. Very good correlation was found between HT on the one hand and ASLO or anti-SK, anti-HA and anti-NADase antibodies on the other hand. HT and anti-DNase B antibodies were less correlated. Haemagglutination titres appear to rise earlier than serological titres of conventional streptococcal antibodies. The haemagglutination test described may be particularly helpful as a rapid serologic indicator of streptococcal infections and more reliable than the titration of ASLO alone, or of any one of anti-enzyme antibodies.
已设计出一种用于一次性U型板的微量血凝试验，用于快速、定量评估人血清中的抗链球菌抗体。使用了经戊二醛处理的新鲜或冻干绵羊红细胞，这些红细胞用A组化脓性链球菌释放的十五种以上细胞外蛋白致敏，包括链球菌溶血素O、脱氧核糖核酸酶、透明质酸酶、链激酶和烟酰胺二核苷酸糖水解酶。对123名健康受试者（“对照组”）和311名有疑似或明显链球菌感染病史的患者的434份血清标本同时测定了血凝和抗链球菌溶血素O（ASLO）滴度。还对两组约100份血清进行了上述四种抗酶抗体的滴定。对照组血清中的血凝滴度（HT）小于800。相比之下，患者标本中的血凝滴度大于800，最高可达12800。一方面发现HT与ASLO或抗SK、抗HA和抗NADase抗体之间有非常好的相关性。HT与抗DNase B抗体的相关性较小。血凝滴度似乎比传统链球菌抗体的血清学滴度上升得更早。所描述的血凝试验作为链球菌感染的快速血清学指标可能特别有用，并且比单独滴定ASLO或任何一种抗酶抗体更可靠。
