Skilton H E, Eperon I C, Rivett A J
Department of Biochemistry, University of Leicester, UK.
FEBS Lett. 1991 Feb 25;279(2):351-5. doi: 10.1016/0014-5793(91)80185-6.
Previous studies have come to different conclusions about the presence of RNA in particles known variously as prosomes, proteasomes or multicatalytic proteinase (MCP). To determine the reason for this, MCP was isolated from rat liver by 4 different purification protocols. One major band of RNA, about 80 nucleotides in length, co-purified in all preparations. The amount of RNA detected was less than one molecule per MCP particle suggesting that there may be more than one population of MCP in rat liver cells.
先前的研究对于在被称为前体颗粒、蛋白酶体或多催化蛋白酶（MCP）的颗粒中是否存在RNA得出了不同的结论。为了确定其原因，通过4种不同的纯化方案从大鼠肝脏中分离出MCP。在所有制备物中共同纯化出一条主要的RNA条带，长度约为80个核苷酸。检测到的RNA量少于每个MCP颗粒一个分子，这表明大鼠肝细胞中可能存在不止一种类型的MCP。
