硼酸和硼酸酯对木豆脲酶的抑制作用。

Boric acid and boronic acids inhibition of pigeonpea urease.

作者信息

Reddy K Ravi Charan, Kayastha Arvind M

机构信息

School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.

出版信息

J Enzyme Inhib Med Chem. 2006 Aug;21(4):467-70. doi: 10.1080/14756360600638147.

DOI:10.1080/14756360600638147

PMID:17059182

Abstract

Urease from the seeds of pigeonpea was competitively inhibited by boric acid, butylboronic acid, phenylboronic acid, and 4-bromophenylboronic acid; 4-bromophenylboronic acid being the strongest inhibitor, followed by boric acid > butylboronic acid > phenylboronic acid, respectively. Urease inhibition by boric acid is maximal at acidic pH (5.0) and minimal at alkaline pH (10.0), i.e., the trigonal planar B(OH)3 form is a more effective inhibitor than the tetrahedral B(OH)4 -anionic form. Similarly, the anionic form of phenylboronic acid was least inhibiting in nature.

摘要

来自木豆种子的脲酶受到硼酸、丁基硼酸、苯硼酸和4-溴苯硼酸的竞争性抑制；4-溴苯硼酸是最强的抑制剂，其次是硼酸>丁基硼酸>苯硼酸。硼酸对脲酶的抑制在酸性pH（5.0）时最大，在碱性pH（10.0）时最小，即三角平面的B(OH)₃形式比四面体的B(OH)₄⁻阴离子形式是更有效的抑制剂。同样，苯硼酸的阴离子形式在本质上抑制作用最小。