大豆脲酶与重金属、矿物酸酸的钠盐、硼酸和硼酸的抑制研究。

Inhibition studies of soybean (Glycine max) urease with heavy metals, sodium salts of mineral acids, boric acid, and boronic acids.

机构信息

School of Biotechnology, Faculty of science, Banaras Hindu University, Varanasi 221005, India.

出版信息

J Enzyme Inhib Med Chem. 2010 Oct;25(5):646-52. doi: 10.3109/14756360903468155.

PMID:20014894

Abstract

Various inhibitors were tested for their inhibitory effects on soybean urease. The K(i) values for boric acid, 4-bromophenylboronic acid, butylboronic acid, and phenylboronic acid were 0.20 +/- 0.05 mM, 0.22 +/- 0.04 mM, 1.50 +/- 0.10 mM, and 2.00 +/- 0.11 mM, respectively. The inhibition was competitive type with boric acid and boronic acids. Heavy metal ions including Ag(+), Hg(2+), and Cu(2+) showed strong inhibition on soybean urease, with the silver ion being a potent inhibitor (IC(50) = 2.3 x 10(-8) mM). Time-dependent inhibition studies exhibited biphasic kinetics with all heavy metal ions. Furthermore, inhibition studies with sodium salts of mineral acids (NaF, NaCl, NaNO(3), and Na(2)SO(4)) showed that only F(-) inhibited soybean urease significantly (IC(50) = 2.9 mM). Competitive type of inhibition was observed for this anion with a K(i) value of 1.30 mM.

摘要

多种抑制剂被测试其对大豆脲酶的抑制作用。硼酸、4-溴苯硼酸、正丁基硼酸和苯硼酸的 K(i)值分别为 0.20±0.05mM、0.22±0.04mM、1.50±0.10mM 和 2.00±0.11mM。硼酸和苯硼酸的抑制作用是竞争性的。重金属离子，包括 Ag(+)、Hg(2+)和 Cu(2+)，对大豆脲酶表现出强烈的抑制作用，其中银离子是一种有效的抑制剂（IC(50) = 2.3 x 10(-8)mM）。时间依赖性抑制研究表明，所有重金属离子都表现出双相动力学。此外，用矿物酸的钠盐（NaF、NaCl、NaNO(3)和 Na(2)SO(4))进行的抑制研究表明，只有 F(-)显著抑制大豆脲酶（IC(50) = 2.9mM）。对于这种阴离子，观察到竞争性抑制，K(i)值为 1.30mM。