Breitenbach J M, Hausinger R P
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
Biochem J. 1988 Mar 15;250(3):917-20. doi: 10.1042/bj2500917.
Urease was purified 800-fold and partially characterized from Proteus mirabilis, the predominant microorganism associated with urinary stones. Boric acid is a rapid reversible competitive inhibitor of urease. The pH-dependence of inhibition exhibited pKa values of 6.25 and 9.3, where the latter value is probably due to the inherent pKa of boric acid. Three boronic acids also were shown to inhibit urease competitively.
脲酶从奇异变形杆菌(与尿路结石相关的主要微生物)中被纯化了800倍并进行了部分特性分析。硼酸是脲酶的一种快速可逆竞争性抑制剂。抑制作用的pH依赖性表现出pKa值为6.25和9.3,其中后一个值可能归因于硼酸固有的pKa。三种硼酸也被证明可竞争性抑制脲酶。