Tungsinmunkong Kobkul, Suwiwat Supaporn, Sriplung Hutcha
Department of Pathology, Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat-Yai, Songkhla 90110, Thailand.
Asian Pac J Cancer Prev. 2006 Jul-Sep;7(3):427-30.
To evaluate the prevalence of high-risk type human papillomavirus (HR-HPV) in preneoplastic lesions and invasive squamous cell carcinoma (SCC) of the cervix uteri in southern Thai women.
A total of 148 formalin-fixed, paraffin-embedded blocks of cervix tissue were retrieved from the files of the Department of Pathology, Prince of Songkla University Hospital. They were classified as negative for intraepithelial lesion (NIL) in 37 cases, low grade lesion (LGL) in 58 cases, high grade lesion (HGL) in 39 cases and SCC in 14 cases. HR-HPV DNA was tested with an Amplicor HPV (Roche Diagnostics) detection kit.
Of the 111 cases, 42 of 58 LGLs (72.4%), 34 of 39 HGLs (87.2%) and 13 of 14 SCCs (92.9%) were positive for HR-HPV DNA. In 37 cases of histologically normal cervix, there were 15 cases that showed the presence of HR-HPV DNA. Applying the HR-HPV results for NILs to the general population, the age standardized incidence rate of HR-HPV infection in the normal Thai population was 12.8%.
HR-HPV DNA can be found in all grades of intraepithelial lesions and carcinoma of the cervix uteri, even in the histologically "normal" looking cervix. These results provide strong evidence for a role in carcinogenesis of the cervix uteri and the existence of a non-productive or latent period of HPV infection.
评估泰国南部女性子宫颈癌前病变和浸润性鳞状细胞癌（SCC）中高危型人乳头瘤病毒（HR-HPV）的感染率。
从宋卡王子大学医院病理科档案中检索出148例福尔马林固定、石蜡包埋的子宫颈组织块。其中37例为上皮内病变阴性（NIL），58例为低级别病变（LGL），39例为高级别病变（HGL），14例为SCC。使用Amplicor HPV（罗氏诊断）检测试剂盒检测HR-HPV DNA。
在111例病例中，58例LGL中有42例（72.4%）、39例HGL中有34例（87.2%）以及14例SCC中有13例（92.9%）的HR-HPV DNA检测呈阳性。在37例组织学正常的子宫颈病例中，有15例检测出HR-HPV DNA。将NILs的HR-HPV检测结果应用于普通人群，泰国正常人群中HR-HPV感染的年龄标准化发病率为12.8%。
在子宫颈各级上皮内病变及癌组织中均可检测到HR-HPV DNA，即使在组织学表现“正常”的子宫颈中也可检测到。这些结果为HR-HPV在子宫颈癌发生过程中的作用以及HPV感染存在非增殖或潜伏期提供了有力证据。
