Mosier-Boss P A
SPAWAR Systems Center San Diego, Code 2373, San Diego, California 92152, USA.
Appl Spectrosc. 2006 Oct;60(10):1148-56. doi: 10.1366/000370206778664699.
Silver and gold colloidal particles can be immobilized on amine-derivatized magnetic microparticles. Once immobilized, the colloidal particles can be reacted with thiols to form a self-assembled monolayer (SAM). Earlier it was shown that the resultant derivatized magnetic microparticles, i.e., capture matrices, are surface-enhanced Raman (SERS) active and that they can be used to extract trace amounts of analyte from an aqueous sample. In this investigation, the optical properties of the capture matrices are examined. Imaging of these magnetic microparticles shows that the immobilized silver/gold colloidal particles exhibit blinking behavior. An increase in the SERS signals due to the SAM and the continuum is observed with constant laser illumination of these magnetic microparticles. Such an increase can be used to improve sensitivity. This increase in signal is attributed to the electromagnetic enhancement mechanism (EEM).
银和金的胶体颗粒可以固定在胺衍生化的磁性微粒上。一旦固定，胶体颗粒可以与硫醇反应形成自组装单分子层（SAM）。早期研究表明，所得的衍生化磁性微粒，即捕获基质，具有表面增强拉曼（SERS）活性，并且可用于从水性样品中提取痕量分析物。在本研究中，对捕获基质的光学性质进行了研究。对这些磁性微粒的成像显示，固定的银/金胶体颗粒表现出闪烁行为。在对这些磁性微粒进行恒定激光照射时，观察到由于SAM和连续体导致的SERS信号增加。这种增加可用于提高灵敏度。信号的这种增加归因于电磁增强机制（EEM）。
