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接种量和培养时间对乳酸菌肉汤微量稀释药敏试验的影响。

Effects of inoculum size and incubation time on broth microdilution susceptibility testing of lactic acid bacteria.

作者信息

Egervärn Maria, Lindmark Hans, Roos Stefan, Huys Geert, Lindgren Sven

机构信息

Microbiology Division, National Food Administration, Box 622, SE-751 26 Uppsala, Sweden.

出版信息

Antimicrob Agents Chemother. 2007 Jan;51(1):394-6. doi: 10.1128/AAC.00637-06. Epub 2006 Oct 23.

DOI:10.1128/AAC.00637-06
PMID:17060527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1797673/
Abstract

Inoculum size and incubation time were varied during broth microdilution testing of the susceptibilities of 35 strains of lactic acid bacteria to six antibiotics. An increase in either parameter resulted in elevated MICs for all species. An inoculum of 3 x 10(5) CFU/ml is recommended to assess the antibiotic susceptibilities of these bacteria by using broth microdilution.

摘要

在对35株乳酸菌对六种抗生素的敏感性进行肉汤微量稀释测试期间，接种量和孵育时间有所变化。任一参数的增加都会导致所有菌种的最低抑菌浓度（MIC）升高。建议使用3×10⁵CFU/ml的接种量，通过肉汤微量稀释法来评估这些细菌的抗生素敏感性。

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本文引用的文献

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