Hu Limin, Ferrara Napoleone, Jaffe Robert B
Center for Reproductive Sciences, University of California, San Francisco, 505 Parnassus Avenue, HSW 1450, San Francisco, CA 94143-0556, USA.
Exp Biol Med (Maywood). 2006 Nov;231(10):1646-52. doi: 10.1177/153537020623101010.
Ascites formation associated with neoplasms is most likely due to increased vascular permeability, a process in which vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) plays a pivotal role. We hypothesized that tumor-derived VEGF/VPF modulates ascites formation through a paracrine effect on both tumor and peritoneal vessels. We investigated human vascular endothelial permeability using a newly developed dual-chamber permeability assay by co-culturing human umbilical vein cells with and without ovarian cancer cell lines (OVCAR-3, Hey-A8, and OCC-1) in the presence or absence of a human VEGF monoclonal antibody and VE-cadherin function-blocking antibody. This method permits determination of mechanisms by which substances released from neoplasms and other sources of vascular endothelial cell secretagogues modulate vascular permeability and likely other pathologic states.
与肿瘤相关的腹水形成很可能是由于血管通透性增加，在这个过程中血管内皮生长因子/血管通透因子（VEGF/VPF）起着关键作用。我们推测肿瘤来源的VEGF/VPF通过对肿瘤和腹膜血管的旁分泌作用来调节腹水形成。我们通过一种新开发的双室通透性测定法来研究人类血管内皮通透性，该方法是在有或无人类VEGF单克隆抗体和VE-钙黏蛋白功能阻断抗体的情况下，将人脐静脉细胞与卵巢癌细胞系（OVCAR-3、Hey-A8和OCC-1）共培养。这种方法能够确定肿瘤及其他血管内皮细胞分泌源释放的物质调节血管通透性以及可能的其他病理状态的机制。
