Liu Y, Gaur V, Turner J E
Department of Neurobiology and Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
Neurosci Res. 1990 Dec;9(3):184-95. doi: 10.1016/0168-0102(90)90003-w.
The opsin-like immunoreactivity in the retinas of C57BL/6J rdle mutant mice has been studied by light-microscopic immunocytochemistry. Positively labeled cells were found in the normal heterozygous and homozygous mutant mouse outer nuclear layer (ONL) as early as postnatal day 3 (PN3). Beginning at PN10, in the retinas of the homozygous mutant mice, labeled photoreceptor cells rapidly decreased in number and disappeared after PN42. In the decreased ONL, remaining opsin-positive cells were labeled at higher density than those of controls. The retinal pigment epithelium was also moderately labeled during the loss of opsin-positive photoreceptor cells. In addition, sparse opsin-immunoreactive cells were demonstrated in the inner nuclear layer (INL) in the retinas of both the mutant and non-dystrophic mice as early as PN10 and are presumed to be ectopic photoreceptor cells. However, these displaced photoreceptor cells disappeared by PN28 in mutants along the same time course as those in the ONL but were still present in the PN28 retina of controls and seemed to be more abundant at later adult ages. There was no difference in the developmental regulation of opsin in the heterozygous and normal controls.
通过光学显微镜免疫细胞化学方法，对C57BL/6J rdle突变小鼠视网膜中的视蛋白样免疫反应性进行了研究。早在出生后第3天（PN3），就在正常杂合子和纯合子突变小鼠的外核层（ONL）中发现了阳性标记细胞。从PN10开始，在纯合子突变小鼠的视网膜中，标记的光感受器细胞数量迅速减少，并在PN42后消失。在变薄的ONL中，剩余的视蛋白阳性细胞的标记密度高于对照组。在视蛋白阳性光感受器细胞减少的过程中，视网膜色素上皮也有中度标记。此外，早在PN10时，在突变小鼠和非营养不良小鼠的视网膜内核层（INL）中都发现了稀疏的视蛋白免疫反应性细胞，推测为异位光感受器细胞。然而，这些移位的光感受器细胞在突变体中在PN28时消失，其时间进程与ONL中的细胞相同，但在PN28的对照小鼠视网膜中仍然存在，并且在成年后期似乎更为丰富。杂合子和正常对照中视蛋白的发育调控没有差异。
