Ishiguro S, Fukuda K, Kanno C, Mizuno K
Cell Struct Funct. 1987 Apr;12(2):141-55. doi: 10.1247/csf.12.141.
Immunoreactive opsin was detectable in the apical portion of normally developing photoreceptor cells on postnatal day 3 by the indirect enzyme-labeled antibody method. Immunoreactivity increased and had extended from the central retina to the periphery by the advanced stages of development. In the rd mutant retinas, accumulated opsin was present in the apical portion and in the outer nuclear layer on postnatal day 8. Immunoreactive opsin mainly was present in the outer nuclear layer by day 14, even being detectable on day 28. No immunoreactivity was present in the remaining cones. Electron microscopic immunocytochemistry confirmed the association of immunoreactive opsin with the persistent rod cell plasma membrane. Molecular weight of immunoreactive opsin in 14-day-old rd mutant mouse retina, as estimated by gel filtration chromatography, was large and did not seem to be degraded. These findings indicate that accumulated rhodopsin continues to function in the plasma membrane because an electroretinogram could be made after day 14 for the rd mutant mouse retina.
出生后第3天，通过间接酶标抗体法可在正常发育的光感受器细胞顶端部分检测到免疫反应性视蛋白。在发育后期，免疫反应性增强并从视网膜中央扩展到周边。在rd突变体视网膜中，出生后第8天顶端部分和外核层存在积累的视蛋白。到第14天，免疫反应性视蛋白主要存在于外核层，甚至在第28天仍可检测到。其余视锥细胞中无免疫反应性。电子显微镜免疫细胞化学证实免疫反应性视蛋白与持续存在的视杆细胞质膜相关。通过凝胶过滤色谱法估计，14日龄rd突变体小鼠视网膜中免疫反应性视蛋白的分子量较大，似乎未降解。这些发现表明，积累的视紫红质继续在质膜中发挥作用，因为rd突变体小鼠视网膜在第14天后仍可记录到视网膜电图。
