蛋白质合成第一步与ppGpp的关系：A(5')ppp(5')Gpp的形成。
Relationship of the first step in protein synthesis to ppGpp: formation of A(5')ppp(5')Gpp.
作者信息
Rapaport E, Svihovec S K, Zamecnik P C
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2653-7. doi: 10.1073/pnas.72.7.2653.
Abstract
In the presence of purified Escherichia coli lysyl-tRNA synthetase [L-lysine:tRNALys ligase (AMP-forming) EC 6.1.1.6], L-lysine, and ATP, addition of the nucleotide ppGpp results in formation of a unique product-A(5')ppp(5') Gpp. The same compound is also formed very rapidly in a cell-free protein-synthesizing system when ppGpp is added. The possible significance of this reaction in the rapid turnover of ppGpp and as a more general mechanism by which an AMP residue is activated and introduced onto a 5'-diphosphorylated species, including the 5'-end of an RNA, is further discussed.
摘要
在纯化的大肠杆菌赖氨酰 - tRNA合成酶[L - 赖氨酸：tRNALys连接酶（形成AMP），EC 6.1.1.6]、L - 赖氨酸和ATP存在的情况下，添加核苷酸ppGpp会导致形成一种独特的产物——A(5')ppp(5')Gpp。当添加ppGpp时，在无细胞蛋白质合成系统中也会非常迅速地形成相同的化合物。本文进一步讨论了该反应在ppGpp快速周转中的可能意义，以及作为一种更普遍的机制，通过该机制AMP残基被激活并引入到5'-二磷酸化的物种上，包括RNA的5'-末端。
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本文引用的文献
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