Oláh I, Antoni F, Csuka I, Törö J
Institute of Anatomy, Department II, Semmelweis University Medical School, Budapest, Hungary.
Acta Physiol Hung. 1990;75(4):293-302.
The emetine effectively abolished the plasma cell population in the chicken's gland of Harder by day 3 of treatment. The plasma cell content regenerated by day 5 following emetine injection, possibly from a metabolically inactive, resting B cell population which was resistant to the emetine treatment. By day 7 extracellular substance in a very large quantity appeared among the plasma cells and epithelial cells which might represent a hyperactive plasma cell secretion. The changes in the circulating antibodies measured by hemagglutination well-correlated with the plasma cell content in the gland of Harder. The gland of Harder (GH) is an accessory lacrimal gland. Its main function is to lubricate the nictitating membrane and keep the surface of the eyeball wet. The presence and regulatory function of cAMP dependent histone kinase was showed. Since it has been published that in chicken the interstitium of this gland contains a proper amount of plasma cells, this observation called the attention of many investigators to study the role of GH in the immune response. The B cell maturation in the chicken GH has been studied. The surface marker studies have proved that beside the B cells the gland contains functionally adequate number of T cell which exert stimulatory effect on B cells to promote their transformation to plasma cells. In addition to T and B cells small number of macrophages also occur. In the 9 H the number of plasma cells is age dependent. At hatch only a few plasma cells occur in the interstitium of the gland but by 3 weeks of age they become predominant. Different isotypes of immunoglobulins are secreted by these plasma cells.
在治疗的第3天，吐根碱有效地消除了鸡哈德氏腺中的浆细胞群体。在注射吐根碱后的第5天，浆细胞含量开始再生，可能来自对吐根碱治疗有抗性的代谢不活跃的静止B细胞群体。到第7天，在浆细胞和上皮细胞之间出现了大量的细胞外物质，这可能代表浆细胞的过度活跃分泌。通过血凝测定的循环抗体变化与哈德氏腺中的浆细胞含量密切相关。哈德氏腺（GH）是一个副泪腺。其主要功能是润滑瞬膜并保持眼球表面湿润。研究显示了cAMP依赖性组蛋白激酶的存在及其调节功能。由于已经发表的研究表明，在鸡中该腺的间质含有适量的浆细胞，这一观察结果引起了许多研究者对研究哈德氏腺在免疫反应中作用的关注。已经对鸡哈德氏腺中的B细胞成熟进行了研究。表面标志物研究证明，除了B细胞外，该腺还含有功能上足够数量的T细胞，这些T细胞对B细胞发挥刺激作用，促进其转化为浆细胞。除了T细胞和B细胞外，还存在少量巨噬细胞。在9日龄时，浆细胞的数量与年龄有关。在孵化时，腺间质中只有少数浆细胞，但到3周龄时它们变得占主导地位。这些浆细胞分泌不同类型的免疫球蛋白。
