Scott T R, Savage M L
Department of Poultry Science, Clemson University, South Carolina 29634-0379, USA.
Microsc Res Tech. 1996 Jun 1;34(2):149-55. doi: 10.1002/(SICI)1097-0029(19960601)34:2<149::AID-JEMT9>3.0.CO;2-N.
Experimentation has been carried out to study proliferation of plasma cells in the chicken Harderian gland (HG) and to determine if a HG factor influences immune cell (i.e., B cell) proliferation. In young chickens, flow cytometric analysis of propidium iodide (PI)-stained plasma cells revealed that the percentages of cells in both the synthetic (S) and mitotic (G2M) phases of the cell cycle were highest between 6 and 9 weeks of age. A pattern of plasma cell depletion and repopulation in the HG was observed following administration of emetine dihydrochloride. At 3 and 5 days posttreatment the plasma cell population decreased, and by 7 days posttreatment repopulation of the gland with plasma cells occurred. This repopulation appeared as a result of plasma cell proliferation within the HG. Anti-5-bromo-2'-deoxyuridine (BrdUrd) staining of frozen sections showed that the numbers of plasma cells incorporating BrdUrd were low at 3 days posttreatment but were as high, or higher than, controls at 5 and 7 days posttreatment. These results were verified with flow cytometric data of PI-stained plasma cells. Data from bursal cell bioassays revealed proliferative activity influenced by a HG factor. Coculture of bursal cells with phorbol dibutyrate and diluted HG supernatants resulted in prolonged and increased proliferation of these cells. It is possible that the HG of chickens supports plasma cell proliferation through the elaboration of a factor which acts like a lymphokine.
已经开展实验来研究鸡哈德氏腺（HG）中浆细胞的增殖情况，并确定HG因子是否影响免疫细胞（即B细胞）的增殖。在幼鸡中，对碘化丙啶（PI）染色的浆细胞进行流式细胞术分析发现，细胞周期的合成期（S期）和有丝分裂期（G2M期）的细胞百分比在6至9周龄时最高。在给予盐酸依米丁后，观察到HG中浆细胞的耗竭和重新聚集模式。在处理后3天和5天，浆细胞数量减少，到处理后7天，腺体中出现浆细胞重新聚集。这种重新聚集似乎是HG内浆细胞增殖的结果。对冰冻切片进行抗5-溴-2'-脱氧尿苷（BrdUrd）染色显示，处理后3天掺入BrdUrd的浆细胞数量较低，但在处理后5天和7天与对照相当或更高。这些结果通过PI染色浆细胞的流式细胞术数据得到验证。法氏囊细胞生物测定的数据显示，增殖活性受HG因子影响。法氏囊细胞与佛波酯二丁酯和稀释的HG上清液共培养导致这些细胞的增殖延长且增加。鸡的HG可能通过分泌一种类似淋巴因子的因子来支持浆细胞增殖。
